Experientia

, Volume 47, Issue 3, pp 261–263 | Cite as

Effects of the pyrimidine-containing cytochrome P-450 inhibitor, fenarimol, on the formation of 20-OH ecdysone in flies

  • P. Kulcsár
  • B. Darvas
  • H. Brandtner
  • J. Koolman
  • H. H. Rees
Research Articles

Summary

The effect of fenarimol, a pyrimidine-containing cytochrome P-450 inhibitor, was tested in vitro on brain-ring gland complexes ofCalliphora vicina (Dipt., Calliphoridae), and on microsomes prepared from the fat body of 0-h wandering stage larvae ofNeobellieria bullata (Dipt., Sacrophagidae). Fenarimol had no influence on the formation of ecdysone, but it was an effective inhibitor of cytochrome P-450-dependent ecdysone 20-monooxygenase.

Key words

Calliphora vicina Neobellieria bullata fenarimol synthesis of 20-hydroxy ecdysone cytochrome P-450 inhibitor microsomal ecdysone 20-monooxygenase 

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References

  1. 2.
    Koolman, J., Insect Biochem.12 (1982) 225.Google Scholar
  2. 3.
    Wilkinson, C. F., in: Bioregulators for Pest Control, vol. 276, pp. 161–176. Ed. P. A. Hedin. ACS Symp. Series, 1985.Google Scholar
  3. 4.
    Matolcsy, G., Varjas, L., and Bordás, B., Acta phytopath. hung.10 (1976) 455.Google Scholar
  4. 5.
    Ragsdale, N. N., and Sisler, H. D., Biochem. biophys. Res. Commun.46 (1972) 2048.PubMedGoogle Scholar
  5. 6.
    Kulcsár, P., Darvas, B., and Varjas, L., Abstr. Int. CNRS Symp., p. 125. Strasbourg 1983.Google Scholar
  6. 7.
    Fónagy, A., Darvas, B., and Kulcsár, P., Abstr., 1st Int. Congr. Dipterology, p. 75. Budapest 1986.Google Scholar
  7. 8.
    Darvas, B., Fónagy, A., and Kulcsár, P., Acta phytopath. ent. hung. (1990) in press.Google Scholar
  8. 9.
    Ohtaki, T., Jap. J. med. Sci. Biol.19 (1966) 97.Google Scholar
  9. 10.
    Darvas, B., Rees, H. H., Kuwano, E., Bélai, I., Matolcsy, G., Timár, T., Hoggard, N., and Tag El-Din, M. H., Abstr. IX. Ecdysone Workshop, Paris 1989.Google Scholar
  10. 11.
    Darvas, B., Abstr. 2nd Int. Cong. Dipterology, Bratislava (1990) in press.Google Scholar
  11. 12.
    Wilkinson, C. F., and Murray, M., Drug Metab. Rev.15 (1984) 897.PubMedGoogle Scholar
  12. 13.
    Bidmon, H. J., Käuser, G., Möbus, P., and Koolman, J., in: Proc. 3rd Neem Conf., Nairobi, pp. 253–271. Eds. H. Schmutterer and K. R.-S. Ascher. GTZ GmbH 1986.Google Scholar
  13. 14.
    Bollenbacher, W. E., Smith, S. L., Wielgus, J. J., and Gilbert, L. I., Nature268 (1977) 660.Google Scholar
  14. 15.
    Hoffmann, J. A., and Hetru, C., in: Endocrinology of Insects, pp. 65–68. Eds G. H. Downer and H. Laufer. Alan R Liss, New York 1983.Google Scholar
  15. 16.
    Rees, H. H., Nova Acta Leopoldina, NF 56255 (1984) 267.Google Scholar
  16. 17.
    Clarke, G. S., Baldwin, B. C., and Rees, H. H., Pest. Biochem. Physiol.24 (1985) 220.Google Scholar
  17. 18.
    Nebert, D. W., and Gonzales, F. J., A. Rev. Biochem.56(1987) 945.Google Scholar
  18. 19.
    Kappler, C., Kabbouh, M., Durst, F., and Hoffmann, J. A., Insect Biochem.16 (1986) 25.Google Scholar
  19. 20.
    Applebaum, S. W., personal communication.Google Scholar
  20. 21.
    Darvas, B., and Zsellér, H. I., Acta phytopath. hung.20 (1985) 341.Google Scholar

Copyright information

© Birkhäuser Verlag 1991

Authors and Affiliations

  • P. Kulcsár
    • 1
  • B. Darvas
    • 1
  • H. Brandtner
    • 2
  • J. Koolman
    • 2
  • H. H. Rees
    • 3
  1. 1.Department of Zoology, Plant Protection InstituteHungarian Academy of SciencesBudapest(Hungary)
  2. 2.Physiologisch-Chemisches Institut der Phillips-UniversitätMarburg(Germany)
  3. 3.Department of BiochemistryUniversity of LiverpoolLiverpool(England)

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