European Journal of Pediatrics

, Volume 151, Issue 11, pp 802–805 | Cite as

Chlorinated hydrocarbons in the bone Marrow of children: Studies on their association with leukaemia

  • J. Scheele
  • M. Teufel
  • K. H. Niessen


Concentrations of chlorinated hydrocarbons and polyclorinated biphenyls (PCB) were determined by capillary column gas chromatography in samples of bone marrow from 38 children with leukaemia (16 samples/pools) and 15 control (5 pools). The highest mean and median concentrations were detected for total PCB (mean=3.568 mg/kg fat basis/median 2.904 mg/kg) followed by the sum of the dichlorodiphenyltrichloroethane metabolites (1.775/1.059 mg/kg), hexachlorobenzene 0.354/0.260 mg/kg), the sum of the hexachlorocyclohexane isomers (0.133/0.093 mg/kg) and dieldrin (0.109/0.063 mg/kg). The CHC and PCB concentrations in bone marrow were two- to threefold higher than in fat tissue. Comparing children with and without leukaemia similar concentrations of CHC and PCB were found.

Key words

Pesticides Chlorinated hydrocarbons Polychlorinated biphenyls Bone marrow Leukaemia 



chlorinated hydrocarbons










polychlorinated biphenyls


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  1. 1.
    Aksoy M, Dincol K, Erdem S, Dincol G (1972) Acute leukemia due to chronic exposure to benzene. Am J Med 52:160–166CrossRefPubMedGoogle Scholar
  2. 2.
    Aksoy M, Erdem S, Dincol G (1974) Leukemia in shoeworkers exposed chronically to benzene. Blood 44:837–841PubMedGoogle Scholar
  3. 3.
    Aksoy M, Erdem S, Dincol G (1976) Types of leukemia in chronic benzene poisoning. A study in thirty-four patients. Acta Haematol (Basel) 55:65–72Google Scholar
  4. 4.
    Ballschmiter K, Zell M (1980) Analysis of polychlorinated biphenyls (PCB) by glass capillary gas chromatography. Fresenius Z Anal Chem 302:20–31CrossRefGoogle Scholar
  5. 5.
    Beck H, Mathar W (1985) Analyseverfahren zur Bestimmung von ausgewaehlten PCB-Einzelkomponenten in Lebensmitteln. Bundesgesundheitsblatt 28:1–12Google Scholar
  6. 6.
    Blair A, Thomas TL (1979) Leukemia among Nebraska farmers: a death certificate study. Am J Epidemiol 110:264–273PubMedGoogle Scholar
  7. 7.
    Cronkite EP, Bullis JE, Inoue T, Drew RT (1984) Benzene inhalation produces leukemia in mice. Toxicol Appl Pharmacol 75:358–361CrossRefPubMedGoogle Scholar
  8. 8.
    Deutsche Forschungsgemeinschaft (1988) Pruefung von Rueckstaenden in Lebensmitteln. Mitteilung XIII. Verlag Chemie, Weinheim, p. 24Google Scholar
  9. 9.
    Dolphin RJ, Willmot FW, Mills FW, Hoogeveen LPJ (1976) Column switching technique in the liquid chromatographic analysis of organochlorine pesticides in milk. J Chromatogr 122:259–268CrossRefPubMedGoogle Scholar
  10. 10.
    Flodin U, Andersson L, Anjou CG, Palm UB, Vikrot O, Axelson O (1981) A case-referent study on acute myeloid leukemia, background radiation and exposure to solvents and other agents. Scand J Work Environ Health 7:169–178Google Scholar
  11. 11.
    Flodin U, Fredrikson M, Axelson O, Perrson B, Hardell L (1986) Background radiation, electrical work and some other exposures associated with acute myeloid leukemia in a case-referent study. Arch Environ Health 41:77–84PubMedGoogle Scholar
  12. 12.
    Flodin U, Frederikson M, Perrson B, Axelson O (1988) Chronical lymphatic leukemia and engine exhaust, fresh wood and DDT: a case-referent study. Br J Ind Med 45:33–38PubMedGoogle Scholar
  13. 13.
    Formi A, Vigliani EC (1974) Chemical leukemogenesis in man. Ser Haematol 7:211PubMedGoogle Scholar
  14. 14.
    Forth W (1980) Hexachlorcyclohexan — Gift in den Lebensmitteln? Dtsch Aerztebl 37:2169–2176Google Scholar
  15. 15.
    Hans HJ (1976) Aplastic anemia associated with gamma-benzene hexachloride. JAMA 236:9–10CrossRefGoogle Scholar
  16. 16.
    Hardell L, Erikson M, Lenner P, Lundgren E (1981) Malignant lymphoma and exposure to chemicals especially organic solvents, chlorophenols and phenoxy acids: a case control study. Br J Cancer 43:169–176PubMedGoogle Scholar
  17. 17.
    IARC/International Agency for Research on Cancer (1979) Hexachlorocyclohexane (technical HCH and Lindane). IARC Monogr Environ Carcinog Risk Chem Hum 20:195–239Google Scholar
  18. 18.
    Infante PF, Epstein SS, Newton WA Jr (1978) Blood dyscrasias and childhood tumors and exposure to chlordane and heptachlor. Scand J Work Environ Health 4:137–150PubMedGoogle Scholar
  19. 19.
    Jedlicka V, Hermanska Z, Smida I, Kauka A (1958) Paramyeloblastic leukemia in two blood cousins after simultaneous contact with Gammexane. Acta Med Scand 161:447–451PubMedGoogle Scholar
  20. 20.
    Loge JP (1965) Aplastic anemia following exposure to benzene hexachloride (lindane). JAMA 193:628–629Google Scholar
  21. 21.
    Maroni M, Colombi A, Arbosti G, Cantoni S, Foa V (1981) Occupational exposure to polychlorinated biphenyls in electrical workers. II. Health effects. Br J Ind Med 38:55–60PubMedGoogle Scholar
  22. 22.
    Mitelman F, Brandt L, Nilson S (1978) Relation among occupational exposure to potential mutagenic, carcinogenic agents, clinical findings, and bone marrow chromosomes in acute nonlymphocytic leukemia. Blood 52:1229–1237PubMedGoogle Scholar
  23. 23.
    Morgan DP, Lin LI, Laikaly HH (1980) Morbidity and mortality in workers occupationally exposed to pesticides. Arch Environ Contam Toxicol 9:349–382CrossRefPubMedGoogle Scholar
  24. 24.
    Scheele JS (1991) Chlorierte Kohlenwasserstoffpestizide und polychlorierte Biphenyle im Knochenmark von gesunden und leukaemiekranken Kindern und Erwachsenen. Thesis, HeidelbergGoogle Scholar
  25. 25.
    Teufel M, Niessen KH, Sartoris J, Brands W, Lochbuehler H, Waag K, Schweizer P, Oelsnitz G von (1990) Chlorinated hydrocarbons in fat tissue: analyses of residues in healthy children, tumor patients and malformed children. Arch Environ Contam Toxicol 19:646–652CrossRefPubMedGoogle Scholar
  26. 26.
    Unger M, Nordley GF (1979) Distribution of PCB and DDT among human tissues. Arch Ind Hyg Toxicol [Suppl] 30:537–543Google Scholar
  27. 27.
    Unger M, Olson J (1980) Organochlorine compounds in the adipose tissue of deceased people with and without cancer. Environ Res 23:257–263CrossRefPubMedGoogle Scholar
  28. 28.
    Vigliani EC (1976) Leukemia associated with benzene exposure. Ann NY Acad Sci 271:143–151PubMedGoogle Scholar
  29. 29.
    Vigliani EC, Forni A (1976) Benzene and leukemia. Environ Res 11:122–127CrossRefPubMedGoogle Scholar
  30. 30.
    Vigliani EC, Saita G (1974) Benzene and leukemia. N Engl J Med 271:872–876Google Scholar
  31. 31.
    West J (1967) Industrial hematologic reaction. Arch. Environ Health 15:37–101Google Scholar
  32. 32.
    Woodcliff P, Conner M, Scopa J (1966) Aplastic anemia associated with insecticides. Med J Anat 1:628–629Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • J. Scheele
    • 1
  • M. Teufel
    • 1
  • K. H. Niessen
    • 1
  1. 1.Children's Hospital MannheimUniversity of HeidelbergMannheimGermany

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