Abstract
Histochemical and immunohistochemical studies performed in only a few cases of sinus histiocytosis with massive lymphoadenopathy (SHML) indicated that SHML cells belong to themacrophage — histiocyte system, though their exact origin is still uncertain. We analyzed the morphological, antigenic and enzymatic characteristics of the histiocyte-like cells in one paediatric case of SHML (also named Rosai-Dorfman disease). The SHML cells expressed the S-100 protein, lectins concanavalin A, peanut agglutinin andmonocyte-macrophage related antigens CD 11c, CD 14, CD 33, CD 68 and LN 5. Reactivity with other anti-macrophage antibodies (MAC 387, lysozyme, alpha-1 anti-chymotrypsin) was variable. The CD1a antigen was present only in scattered cells, whereas HLA-DR and the HLA-DR associated invariant chain were absent. Cytochemistry demonstrated an intense activity of acid phosphatase and non specific esterase of SHML cells. A large amount of medium sized mononuclear cells were located in the sinuses and intersinusoidal tissue. Our findings suggest that SHML cells have intermediate features between phagocytes and Langerhans cells/interdigitating reticulum cells. The heterogeneity of marker expression on SHML cells might be related to the local content of factors (e.g., cytokines), capable of modulating the phenotype of monocyted and derived cells.
Similar content being viewed by others
Abbreviations
- ConA:
-
concanavalin agglutinin
- MoAb:
-
monoclonal antibody
- PNA:
-
peanut agglutinin
- SHML:
-
sinus histiocytosis with massive lymphoadenopathy
References
Azoury FJ, Reed RJ (1966) Histiocytosis: report of an unusual case. N Engl J Med 274:928–930
Bhoopat L, Turner RR, Stathopoulos E, et al (1988) Immunohistochemical characterization of two new monoclonal antibodies (LN 4, LN 5) reactive with human macrophage subsets and derived malignancies in B5-fixed, paraffin embedded tissues. Blood 71:1079–1085
Bonetti F, Chilosi M, Menestrina F, et al (1987) Immunohistological analysis of Rosai-Dorfman histiocytosis. A disease of S-100 + CD 1-histiocytes. Virchows Arch A 411:129–135
Cordell JL, Falini B, Erber WN, et al (1984) Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem 32:219–229
Destombes P (1965) Adenitis with lipid excess, in children or young adults, seen in the Antilles and Mali (4 cases). Bull Soc Pathol Exot Filiales 58:1169–1175
Eisen RN, Buckley PJ, Rosai J (1990) Immunophenotypic characterization of sinus histiocytosis with massive lymphoadenopathy (Rosai-Dorfman disease). Semin Diagn Pathol 7:74–82
Foucar K, Foucar E (1990) The mononuclear phagocyte and immunoregulatory system: evolving concept. Semin Diagn Pathol 7:4–18
Foucar E, Rosai J, Dorman RF (1990) Sinus histiocytosis with massive lymphoadenopathy (Rosai-Dorfman disease). Semin Diagn Pathol 7:19–73
Franklin WA, Mason DY, Pulford K, Falini B, Bliss E, Gatter KC, Stein H, Clarke LC, Mac Gee J (1986) Immunohistological analysis of human mononuclear phagocytes and dendritic cells by using monoclonal antibodies. Lab Invest 54:32
Goldstein J, Braveman M, Scalafia C, Buckley P (1988) The phenotype of human placental macrophages and its variation with gestational age. Am J Pathol 133:648
Lasser A (1983) The mononuclear phagocytic system: a review. Hum Pathol 14:108–126
Lennert K (1961) Lymphoknoten bey Sklerom. In: Lubarsch O, Henke F (eds) Handbuch der Speziellen Pathologischen Anatomie und Histologie, Vol. 1, Part 3/Band A. Springer Verlag, Berlin Heidelberg New York, pp 504–509
Lopez P, Estes ML (1989) Immunohistochemical characterization of the histiocytes in sinus histiocytosis with massive lymphoadenopathy: analysis of an extranodal case. Hum Pathol 20:711
Maennie DL, Grierson HL, Gnarra DG, Weisenburger DD (1991) Sinus histiocytosis with massive lymphoadenopathy: a spectrum of disease associated with immune dysfunction. Pediatr Pathol 11:399–412
Marie J, Bernard J, Nezelof C, et al (1966) Chronic adenopathy with reticulo-histiocyte proliferation and lipid overloading. Ann Pediatr 42:2689–2697
Murphy GF, Messadi D, Fonferko E, Hancock WW (1986) Phenotypic transformation of macrophages to Langerhans cells in the skin. Am J Pathol 123:401–406
Pulford KA, Rigney EM, Micklem KJ, Jones M, Stross WP (1989) KP 1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections. J Clin Pathol 42:414–421
Ree HJ, Kadin ME (1986) Peanut agglutinin. A useful marker for histiocytosis X and interdigitating reticulum cells. Cancer 57:282–287
Roholl PJM, Kleyne J, Pijpers HW, Van Unnik JAM (1985) Comparative immunohistochemical investigation of markers for malignant histiocytes. Hum Pathol 16:763–771
Rosai J, Dorfman RF (1969) Sinus histiocytosis with massive lymphoadenopathy. A newly recognized benign clinicopathological entity. Arch Pathol 87:63
Rosai J, Dorfman RF (1972) Sinus histiocytosis with massive lymphoadenopathy: a pseudolymphomatous benign disorder. Analysis of 34 cases. Cancer 30:1174–1188
Shi ZR, Itzkowitz SH, Kim YS (1988) A comparison of three immunoperoxidase techniques for antigen detection in colorectal carcinoma tissues. J Histochem Cytochem 36:317–322
Sterberger LA (1986) Immunocytochemistry. 3rd edn. John Wiley and Sons, New York
Testa U, Ferbus D, Gabbianelli M, et al. (1988) Effect of endogenous and exogenous interferons on the differentiation of human monocyte cell line U 937. Cancer Res 48:82–88
Wood GS, Turner RR, Shiurba RA, Eng L, Warnke RA (1985) Human dendritic cells and macrophages. In situ immunophenotypic definition of subsets that exhibit specific morphologic and microenvironmental characteristics. Am J Pathol 119:73–82
Zwaldo G, Brocker EB, Von Bassewitz DB, et al. (1985) A monoclonal antibody to a differentiation antigen present on mature human macrophages and absent from monocytes. J Immunol 137:512:518
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Paulli, M., Locatelli, F., Kindl, S. et al. Sinus histiocytosis with massive lymphoadenopathy (Rosai-Dorfman disease). Clinico-pathological analysis of a paediatric case. Eur J Pediatr 151, 672–675 (1992). https://doi.org/10.1007/BF01957571
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01957571