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Experientia

, Volume 45, Issue 4, pp 365–367 | Cite as

Endocytosis and inositol hexakisphosphate levels inras transformants ofDictyostelium discoideum amoebae

  • G. Klein
  • J. -B. Martin
  • M. Satre
  • C. Reymond
Article

Summary

Fluid-phase pinocytosis kinetics and lysosomal enzyme secretion parameters were measured inDictyostelium discoideum amoebae constructed from strain AX3 by transformation with a multicopy plasmid carrying either a normalras gene (ras-Gly12), a mutatedras gene (ras-Thr12) or by the vector carrying the geneticin resistance gene only (pDNEO2). It was found that the pinocytosis rate and extent as well as the lysosomal enzyme secretion were slightly different in the three strains. These changes, however, were related to minor modifications of the cellular volumes. The overall concentration of inositol hexakisphosphate was similar in the three strains.

Key words

Fluid-phase pinocytosis enzyme secretion inositol hexakisphosphate ras genes Dictyostelium discoideum amoebae 

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Copyright information

© Birkhäuser Verlag Basel 1989

Authors and Affiliations

  • G. Klein
    • 1
  • J. -B. Martin
    • 2
  • M. Satre
    • 1
  • C. Reymond
    • 3
  1. 1.DRF/LBIO/Biochimie (UA 1130 CNRS)Grenoble cedex(France)
  2. 2.DRF/SPh/Résonance Magnétique en Biologie et MédecineCentre d'Etudes NucléairesGrenoble cedex(France)
  3. 3.Swiss Institute for Experimental Cancer Research (ISREC)Epalinges(Switzerland)

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