Endocytosis and inositol hexakisphosphate levels inras transformants ofDictyostelium discoideum amoebae
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Fluid-phase pinocytosis kinetics and lysosomal enzyme secretion parameters were measured inDictyostelium discoideum amoebae constructed from strain AX3 by transformation with a multicopy plasmid carrying either a normalras gene (ras-Gly12), a mutatedras gene (ras-Thr12) or by the vector carrying the geneticin resistance gene only (pDNEO2). It was found that the pinocytosis rate and extent as well as the lysosomal enzyme secretion were slightly different in the three strains. These changes, however, were related to minor modifications of the cellular volumes. The overall concentration of inositol hexakisphosphate was similar in the three strains.
Key wordsFluid-phase pinocytosis enzyme secretion inositol hexakisphosphate ras genes Dictyostelium discoideum amoebae
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