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Experientia

, Volume 45, Issue 4, pp 355–356 | Cite as

Action of juvenile hormone on the follicle cells ofRhodnius prolixus: Evidence for a novel regulatory mechanism involving protein kinase C

  • V. L. Sevala
  • K. G. Davey
Article

Summary

Juvenile hormone (JH) is known to act on the membranes of the follicle cells ofRhodnius, activating a specific Na+, K+-ATPase. This leads to a decrease in volume of the cells and the appearance of spaces between them (patency). The addition of an inhibitor of protein kinase C, 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H-7), to the medium in vitro inhibits the action of JH on the follicle cells. PDBU (phorbol-12,13-dibutyrate) mimics the action of JH in vitro and the response of the follicle cells to, PDBU is blocked by ouabain. It is concluded that the activation of protein kinase C is a required step in the chain of events leading to activation of the JH-dependent ATPase and set in train by the binding of JH to the membrane.

Key words

Juvenile hormone protein kinase C Na+, K+-ATPase follicle cells patency Rhodnius 

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Copyright information

© Birkhäuser Verlag Basel 1989

Authors and Affiliations

  • V. L. Sevala
    • 1
  • K. G. Davey
    • 1
  1. 1.Department of BiologyYork UniversityNorth YorkCanada

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