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Determination of atropine in plasma by a direct radioreceptor assay

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Abstract

A highly sensitive radioreceptor assay for the anticholinergic atropine was developed and could be applied directly to plasma samples obtained from mini-pigs without any clean-up. Plasma samples were collected during 6 h after atropine was administered intravenously or endobronchially. The endobronchial plasma concentration-time curves were characterized by a very rapid rise of the concentration but with a subsequent much slower decrease than after intravenous administration. This indicates an initially high as well as prolonged uptake from the lungs.

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Authors and Affiliations

  1. Groningen Centre for Drug Research, State University of Groningen, Ant. Deusinglaan 2, 9713, AW Groningen, the Netherlands

    K. Ensing & R. A. De Zeeuw

  2. Institute for Anaesthesiology, University of Bonn, Sigmund Freud Strasse 25, 5300, Bonn 1, Germany

    U. Hörnchen, J. Schüttler & H. Stoeckel

Authors
  1. K. Ensing
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  2. R. A. De Zeeuw
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  3. U. Hörnchen
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  4. J. Schüttler
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  5. H. Stoeckel
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Ensing, K., De Zeeuw, R.A., Hörnchen, U. et al. Determination of atropine in plasma by a direct radioreceptor assay. Pharmaceutisch Weekblad Scientific Edition 9, 321–323 (1987). https://doi.org/10.1007/BF01956512

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  • DOI: https://doi.org/10.1007/BF01956512

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