Experientia

, Volume 50, Issue 9, pp 833–836 | Cite as

Inhibition of glucose transport in human erythrocytes by 2,3-dioxoindole (Isatin)

  • M. L. Mousavi Gargari
  • R. C. Bansal
  • K. Singh
  • A. Mahmood
Research Articles

Abstract

10 mM isatin (2,3-dioxoindole) inhibited glucose influx into human erythrocytes by over 30%. The inhibition is of the competitive type, where the affinity constant (Kt) was increased from 5.71 (control) to 11.11 mM in the presence of isatin with no change in Vmax (130 nmol/min/ml packed cells). The observed inhibition of sugar transport by isatin was not mediated through membrane−SH groups accessible to iodoacetate, iodoacetamide, DTNB, DNP or sodium arsenite. Isatin inhibited sugar transport in the presence of 2 mM harmaline, an alkaloid inhibitor of Na+, K+−ATPase activity. The inhibition was non additive which suggests that these two compounds interact with the same or a similar site on the erythrocyte membrane.

Key words

Isatin glucose transport human erythrocytes competitive inhibition 

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Copyright information

© Birkhäuser Verlag 1994

Authors and Affiliations

  • M. L. Mousavi Gargari
    • 1
  • R. C. Bansal
    • 1
  • K. Singh
    • 2
  • A. Mahmood
    • 1
  1. 1.Department of BiochemistryPanjab UniversityPanjabIndia
  2. 2.Department of GastroenterologyInstitute of Medical Education and ResearchChandigarh(India)

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