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Comparison of DMSO and glycerol as cryoprotectants for ascites tumor cells

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Summary

Mouse Ehrlich ascites and rat D23 ascites tumors were stored in liquid nitrogen under identical conditions for up to 3 years. Cell viability (trypan blue exclusion) and transplantability of both tumors in animals remained virtually unaffected if preserved in 10% DMSO containing medium, whereas, cells preserved in 10% glycerol failed to produce lethal tumors in rodents.

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Author notes

  1. S. A. Shah

    Present address: Radiopharmaceutical Research Medical Diagnostics Division, New England Nuclear, 331 Treble Cove Road, 01862, N. Billerica, MA, USA

Authors and Affiliations

  1. Cancer Research Laboratory, Department of Chemical Engineering, Carnegie-Mellon University, 15213, Pittsburgh, Pennsylvania, USA

    S. A. Shah

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  1. S. A. Shah
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Additional information

This work was performed at the Cancer Research Unit, Royal Victoria Infirmary, Newcastle Upon Tyne, England. I thank John Geggie for expert technical assistance; to the North of England Council of the Cancer Research Campaign and the National Science Foundation (ENG-78-25432) for financial support; and to Dr John A. Dickson for advice and encouragement. Also, I am indebted to Professor Rakesh K. Jain for his support.

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Shah, S.A. Comparison of DMSO and glycerol as cryoprotectants for ascites tumor cells. Experientia 39, 327–328 (1983). https://doi.org/10.1007/BF01955331

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