, Volume 39, Issue 3, pp 327–328 | Cite as

Comparison of DMSO and glycerol as cryoprotectants for ascites tumor cells

  • S. A. Shah
Short Communications


Mouse Ehrlich ascites and rat D23 ascites tumors were stored in liquid nitrogen under identical conditions for up to 3 years. Cell viability (trypan blue exclusion) and transplantability of both tumors in animals remained virtually unaffected if preserved in 10% DMSO containing medium, whereas, cells preserved in 10% glycerol failed to produce lethal tumors in rodents.


Nitrogen Tumor Cell Glycerol DMSO Cell Viability 
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Copyright information

© Birkhäuser Verlag 1983

Authors and Affiliations

  • S. A. Shah
    • 1
  1. 1.Cancer Research Laboratory, Department of Chemical EngineeringCarnegie-Mellon UniversityPittsburghUSA

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