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Lactate dehydrogenase activity in cell subpopulations of 7,12-dimethylbenz(a)anthracene-induced mammary tumors

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Summary

Heterogeneity of cells in a solid tumor prevents direct assessment of enzyme activity of cell subpopulations by conventional homogenization techniques. Lactate dehydrogenase (LDH) activity before and after ovariectomy and following estrogen supplementation in density-defined cell subpopulations from DMBA-induced mammary tumors was determined. Different levels of LDH activity were found in different cell subpopulations. After ovariectomy the level of LDH activity declined. Restoration of the circulatory estrogen level resulted in increased enzyme activity. The highest level of LDH was found in a band which consisted mainly of poorly differentiated cells. This cell subpopulation also tended to be more responsive to endocrine manipulation.

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Authors and Affiliations

  1. Department of Anatomy, and Regional Cancer Center, University of Louisville, Health Sciences Center, 40292, Louisville, Kentucky, USA

    A. R. Safa & M. T. Tseng

Authors
  1. A. R. Safa
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  2. M. T. Tseng
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Supported in part by: American Cancer Society grant DPT-100A and a grant from the Graduate School, University of Louisville.

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Safa, A.R., Tseng, M.T. Lactate dehydrogenase activity in cell subpopulations of 7,12-dimethylbenz(a)anthracene-induced mammary tumors. Experientia 39, 283–285 (1983). https://doi.org/10.1007/BF01955306

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  • DOI: https://doi.org/10.1007/BF01955306

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