Experientia

, Volume 39, Issue 3, pp 276–278 | Cite as

Disassociation of oogonia formation from oospore production inPhytophthora cactorum by sterols with varying side chain lengths

  • W. David Nes
  • W. R. Nes
Short Communications

Summary

A series of 20(R)-n-alkylpregn-5-en-3β-ols with 0 to 9 (but not with 12) C-atoms in the alkyl group induced formation of oogonia inPhytophthora cactorum. However, maturation of the oogonia into germinating oospores occurred only when the alkyl group had 4–7 C-atoms.

Keywords

Germinate Chain Length Alkyl Group Side Chain Length Oospore Production 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 2.
    McCorkindale, N. J., Hutchinson, S. A., Pursey B. A., Scott, W. J., and Wheeler, R., Phytochemistry8 (1969) 861.Google Scholar
  2. 3.
    Elliott, C. G., Adv. Microbiol. Physiol.15 (1977) 121.Google Scholar
  3. 4.
    Nes, W. D., Saunders, G. A., and Heftmann, E., Lipids17 (1982) 178.Google Scholar
  4. 5.
    Elliott, C. G., J. gen. Microbiol.115 (1979) 117.Google Scholar
  5. 6.
    Elliott, C. G., Hendrie, M. R., and Knights, B. A., J. gen. Microbiol. (1966).Google Scholar
  6. 7.
    Haskins, R. H., Tulloch, A. P., and Micetich, R. G., Can. J. Microbiol.10 (1964) 187.Google Scholar
  7. 8.
    Elliott, C. G., and Sansome, E., J. gen. Microbiol.98 (1977) 141.Google Scholar
  8. 9.
    All of the sterols used in this study except cholesterol were prepared by J. M. Joseph, Ph.D. Dissertation, 1980, Drexel University. The chromatographic and mass spectral properties of the sterols reisolated fromP. cactorum were similar to those reported by Joseph. See also Nes, W. R., Joseph, J. M., Landrey, J. R., and Conner, R. L., J. biol. Chem.255 (1980) 11815. Procedures for isolation and quantitation are to be found in Nes, W. D., Sanders, G. A., and Heftmann, E., Lipids16 (1981) 744. The control cultures were found to contain small amounts of cholesterol by GLC and GLC-MS as was the Difco agar (derived from red algae which synthesize cholesterol). Thus, the occurrence of a few oogonia in the control is presumably due to the remnants of cholesterol left in the agar after its industrial processing.Google Scholar
  9. 10.
    Nes, W. R., Adler, J. H., Billheimer, J. T., Erickson, K., Joseph, J. M., Landrey, J. R., Marcaccio-Joseph, R., Ritter, K. S., and Conner, R. L., Lipids17 (1982) 257 and references cited therein.Google Scholar
  10. 11.
    Erickson, K., and Nes, W. R., Proc. natl Acad. Sci. USA79 (1982) 4873.Google Scholar
  11. 12.
    Barrow, S. E., and McMorris, T. C., Lipids17 (1982) 383.Google Scholar

Copyright information

© Birkhäuser Verlag 1983

Authors and Affiliations

  • W. David Nes
    • 1
    • 2
  • W. R. Nes
    • 1
    • 2
  1. 1.Plant Physiology and Chemistry Research UnitARS/USDABerkeleyUSA
  2. 2.Department of Biological SciencesDrexel UniversityPhiladelphiaUSA

Personalised recommendations