Skip to main content
SpringerLink
Log in

A nonenzymic oxygen uptake and its implications in the assay of 4-hydroxyphenylpyruvate dioxygenase by an oxygen electrode

  • Short Communications
  • Published:
Experientia Aims and scope Submit manuscript

Summary

The oxygen electrode provides a rapid, convenient assay for 4-hydroxyphenylpyruvate dioxygenase. However, due to the occurrence of a nonenzymic oxygen consumption when reducing agents were mixed witha,a′-dipyridyl, its use is restricted to species which do not require reducing agents or necessitates the addition of catalase to the reaction mixture.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Evans, P. J., Biochim. biophys. Acta677 (1981) 433.

    Article  CAS  PubMed  Google Scholar 

  2. Yamamota, Y., Toyoshima, R., and Muramatsu, K., Agric. Biol. Chem.43 (1979) 585.

    Google Scholar 

  3. Goswami, M. N. D., Biochim. biophys. Acta85 (1964) 390.

    CAS  PubMed  Google Scholar 

  4. Zannoni, V. G., and La Du, B. N., J. biol. Chem.234 (1959) 2925.

    Article  CAS  PubMed  Google Scholar 

  5. Goodwin, B. L., and Werner, E. G., Experientia29 (1973) 523.

    Article  CAS  PubMed  Google Scholar 

  6. Goodwin, B. L., in: Tyrosine catabolism, p. 17. University Press, Oxford 1972.

    Google Scholar 

  7. Knox, W. E., and Le May-Knox, M., Biochem. J.49 (1951) 686.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Houk, T. W., and Ue, K., Analyt. Biochem.62 (1974) 66.

    Article  CAS  PubMed  Google Scholar 

  9. Delieu, T., and Walker, D. A., New Phytol.71 (1972) 201.

    Article  CAS  Google Scholar 

  10. La Du, B. N., and Zannoni, V. G., J. biol. Chem.217 (1955) 777.

    Article  PubMed  Google Scholar 

  11. Fellman, J. H., Fujita, T. S., and Roth, E. S., Biochim. biophys. Acta284 (1972) 90.

    Article  CAS  PubMed  Google Scholar 

  12. Goodwin, B. L., Analyt. Biochem.47 (1972) 302.

    Article  CAS  PubMed  Google Scholar 

  13. Taniguchi, K., Kappe, T., and Armstrong, M. D., J. biol. Chem.239 (1964) 3389.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biochemistry Laboratory, Department of Zoology, University of Oxford, South Parks Road, OX1 3PS, Oxford, UK

    P. J. Evans

  2. Department of Biochemistry, University of Nottingham Medical School, Queen's Medical Centre, NG7 2UH, Nottingham, UK

    P. J. Evans

Authors
  1. P. J. Evans
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Evans, P.J. A nonenzymic oxygen uptake and its implications in the assay of 4-hydroxyphenylpyruvate dioxygenase by an oxygen electrode. Experientia 39, 274–275 (1983). https://doi.org/10.1007/BF01955300

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01955300

Keywords

Search

Navigation