Abstract
Fetal bovine serum (10%) markedly increased ras transcript levels in growth-arrested (Go-synchronized) smooth muscle cells by 8 h. This elevation was maintained for up to 18 h and returned, to pre-stimulation levels within 24 h. Challenge of quiescent cells with serum in the presence of dibetyryl cyclic AMP (1–100 μM), a growth inhibitor for smooth muscle cells, attenuated serum-induced elevation of c-Ha-ras in a dose dependent fashion and preventod progression of the cells into S phase. These results demonstrate that expression of c-Ha-ras in rat aortic smooth muscle cells is cell-cycle dependent and that cAMP prevents the induction of this protooncogene by serum.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literatur
Barrett, T. B., and Benditt, E. P., Proc. natl Acad. Sci. USA84 (1987) 1099.
Kim, H. K., Kim, J. W., Zilberstein, A., Morgolis, B., Kim, J. G., Schlessinger, J., and Rhee, S. G., Cell65 (1991) 435.
Rake, J. B., Quinones, M. A., and Faller, O. U., J. biol. Chem.266 (1991) 5348.
Imler, J. L., Schatz, C., Wasylyk, C., Chatton, B., and Wasylyk, B. A., Nature322 (1988) 275.
Galien, R., Mercier, G., Garcette, M., and Emanoil-Ravier, R., Oncogene6 (1991) 849.
Cox, L. R., Murphy, S. K., and Ramos, K. S., Exp. mol. Path.53 (1990) 52.
Ramos, K. S., and Cox, K. R., in: Meth. Toxic. Eds A. Tyson and J. M. Frazier, (in press).
Chomczynski, P., and Sacchi, N., Anal. biochem.162 (1987) 156.
Tate, E. H., Wilder, M. E., Cram, L. S., and Wharton, W., Cytometry4 (1983) 211.
Bailey, J. L, in: Techniques in Protein Chemistry, p. 341. Ed. J. Leggett. 1967.
Campsi, J., Gray, H. E., Pardee, A. B., Dean, M., and Sonenshein, G. E., Cell36 (1984) 241.
Mulcahy, L. S., Smith, M. R., and Stacey, D. W., Nature313 (1985) 241.
Trepel, J. B., Colamonici, O. R., Kelly, K., Schwab, G., Watt, R. A. Sausville, E. A., Jatle, E. S., and Neckers, L. M., Mol. cell. Biol.7 (1987) 2644.
Cohr, J. R., Torelli, S., Prasad, K. N., Prasad, J. E., and Sharma, O. K., Int. J. dev. Neurosci.9 (1991) 187.
Nakabeppu, Y., Ryder, K. and Nathans, D., Cell55 (1985) 907.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sadhu, D.N., Ramos, K.S. Cyclic AMP inhibits c-Ha-ras protooncogene expression, and DNA synthesis in rat aortic smooth muscle cells. Experientia 49, 567–570 (1993). https://doi.org/10.1007/BF01955165
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01955165