, Volume 45, Issue 2, pp 201–202 | Cite as

A bacterial extracellular polysaccharide which enhances the attachment, ofAgrobacterium tumefaciens to the plant cell surface

  • S. Kawai
  • A. Kobayashi
  • K. Kawazu
Short Communications


Intensive screening, of soil microbial cultures for agglutinating activity ofAgrobacterium tumefaciens resulted in the discovery of a bacterium,Xanthobacter sp. KB-3001, which produced an agglutinin ofA. tumefaciens. This compound is an acidic polysaccharide consisting of glucose and galacturonic acid in the ratio 4∶1. This compound assists the attachment ofA. tumefaciens to plant cells and promotes crown gall formation, owing to its affinity to bothA. tumefaciens and plant cells.

Key words

Agrobacterium tumefaciens agglutination extracellular polysaccharide Xanthobacter sp. cell attachment 


  1. 2.
    Lippincott, B. B., and Lippincott, J. A., J. Bact.97 (1969) 620.PubMedGoogle Scholar
  2. 3.
    Anand, V. K., Pueppke, S. G., and Heberlein, G. T., Plant Physiol.59 (1977) S-109.Google Scholar
  3. 4.
    Sen, P., Chatterjee, G., Kumar, P. M., and Sen, S. K., Indian J. exp. Biol.24 (1986) 153.Google Scholar
  4. 5.
    Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., and Smith, F., Analyt. Chem.28 (1956) 350.CrossRefGoogle Scholar
  5. 6.
    Taylor, R. L., and Conrad, H. E., Biochemistry11 (1972) 1383.CrossRefPubMedGoogle Scholar
  6. 7.
    Nagata, T., and Takebe, I., Planta92 (1970) 301.CrossRefGoogle Scholar
  7. 8.
    Haigler, C. H., Brown, R. M. Jr, and Benziman, M., Science210 (1980) 903.PubMedGoogle Scholar
  8. 9.
    Douglas, C. J., Halperin, W., and Nester, E. W., J. Bact.152 (1982) 1265.PubMedGoogle Scholar
  9. 10.
    Anand, V. K., and Heberlein, G. T., Am. J. Bot.64 (1977) 153.Google Scholar
  10. 11.
    Glogowski, W., and Galsky, A. G., Plant Physiol.61 (1978) 1031.Google Scholar
  11. 12.
    Galsky, A. G., and Wilsey, J. P., Plant Physiol.65 (1980) 184.Google Scholar

Copyright information

© Birkhäuser Verlag Basel 1989

Authors and Affiliations

  • S. Kawai
    • 1
  • A. Kobayashi
    • 1
  • K. Kawazu
    • 1
  1. 1.Laboratory of Bioresources Chemistry, Department of Agricultural ScienceOkayama UniversityOkayama(Japan)

Personalised recommendations