Skip to main content
SpringerLink
Log in

Comparison of retinal pigment epithelium cell preparations from the bovine eye

  • Research Articles
  • Published:
Experientia Aims and scope Submit manuscript

Summary

Retinal pigment epithelium (RPE) cells were collected from, bovine eyes using a new method. The cells were harvested by vortexing the RPE and underlying choroid in 0.05 M citrate phosphate buffer, pH 5. RPE cells recovered by this method were compared to a standard method by microscopic examination of cell integrity, estimation of total protein, and assay of 11-cis and all-trans retinyl ester hydrolase (REH) activities. Results suggest that this method collects RPE cells of good integrity and with a significantly higher protein yield than the conventional method. Additionally, a much higher retinyl ester hydrolase activity was noted. Therefore we propose that this procedure offers a new and convenient method in the collection of RPE proteins for certain purposes such as enzyme purification.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Clark, V. M., The Retina, Part II. Academic Press, New York 1986.

    Google Scholar 

  2. Benjamin-Henkind, J. V., and Zinn, K. M., in: The Retinal Pigment Epithelium, p. 3. Eds M. F. Marmor and K. MN. Zinn. Harvard University Press, Cambridge 1979.

    Google Scholar 

  3. Steinberg, R. H., and Miller, S. S., in: The Retinal Pigment Epithelium, p. 205. Eds Marmor and K. M. Zinn, Harvard University Press, Cambridge 1979.

    Google Scholar 

  4. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. biol. Chem.193 (1951) 265.

    Article  CAS  PubMed  Google Scholar 

  5. Rodriguez, K. A., and Tsin, A. T. C., Am. J. Physiol.256 (1988) 255.

    Google Scholar 

  6. Berman, E. R., in: The Retinal Pigment Epithelium, p. 83. Eds M. F. Marmor and K. M. Zinn, Harvard University Press, Cambridge 1979.

    Google Scholar 

  7. Blaner, W. S., Das, S. R., Gouras, P., and Flood, M. T., J. biol. Chem.262 (1987) 53.

    Article  CAS  PubMed  Google Scholar 

  8. Malsbury, D. W., Sanders-Sanchez, S. R., and Tsin, A. T. C., Invest. Ophthal. Vis. (Suppl).30 (1989) 285.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Life Sciences, The University of Texas at San Antonio, 78285, San Antonio, Texas, USA

    S. R. Sanders-Sanchez, D. Malsbury & A. T. C. Tsin

Authors
  1. S. R. Sanders-Sanchez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. Malsbury
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. T. C. Tsin
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sanders-Sanchez, S.R., Malsbury, D. & Tsin, A.T.C. Comparison of retinal pigment epithelium cell preparations from the bovine eye. Experientia 46, 499–500 (1990). https://doi.org/10.1007/BF01954245

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01954245

Key words

Search

Navigation