, Volume 46, Issue 5, pp 492–495 | Cite as

Survival of BSC-1 cells through the maintenance of cell volume brought about by epidermal growth factor depends on attachment to the substratum

  • Y. Shiba
  • Y. Kanno
Research Articles


Addition of epidermal growth factor to culture medium without calf serum suppressed the increase in cell volume and then enhanced the survival of BSC-1 cells attached to culture dishes. However, these effects of epidermal growth factor were not observed in the case of cells on dishes coated with heat-denatured bovine serum albumin.

Key words

BSC-1 cells cell survival cell volume cell adhesion epidermal growth factor 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Hutchings, S. E., and Sato, G., Proc. natl Acad. Sci. USA75 (1978) 901.Google Scholar
  2. 2.
    Okigaki, T., Kessous, A., and Simard, R., Proc Japan Acad.56 (1980) 206.Google Scholar
  3. 3.
    Sievers, J., Hausmann, B., Unsicker, K., and Berry, M., Neurosci. Lett.76 (1987) 157.Google Scholar
  4. 4.
    Anderson, K. J., Dam, D., Lee, S., and Cotman, C. W., Nature, Lond.332 (1988) 360.Google Scholar
  5. 5.
    McGuire, J. C., and Greene, L. A., J. biol. Chem.254 (1979) 3363.Google Scholar
  6. 6.
    Shiba, Y., Yamada, S., and Kanno, Y., Cell Struct. Funct.14 (1989) 473.Google Scholar
  7. 7.
    Shiba, Y., and Kanno, Y., Cell Struct. Funct.14 (1989) 675.Google Scholar
  8. 8.
    Moolenaar, W. H., Yarden, Y., Laat, S. W. D., and Schlessinger, J., J. biol. Chem.257 (1982) 8502.Google Scholar
  9. 9.
    Owen, N. E., and Villereal, M. L., J. Cell Physiol.117 (1983) 23.Google Scholar
  10. 10.
    Skaper, S. D., and Varon, S., Brain Res.163 (1979) 89.Google Scholar
  11. 11.
    Liepins, A., and Younghusband, H. B., Exp. Cell Res.169 (1987) 385.Google Scholar
  12. 12.
    Levinson, C., J. Membrane Biol.100 (1987) 183.Google Scholar
  13. 13.
    Roy, G., and Sauve, R., J. Membrane Biol.100 (1987) 83.Google Scholar
  14. 14.
    Neville, M. E. J. Immun. Meth.99 (1987) 77.Google Scholar
  15. 15.
    Bank, H. L., In Vitro24 (1988) 266.Google Scholar
  16. 16.
    Shiba, Y., and Kanno, Y., Hiroshima J. med. Sci.28 (1979) 31.Google Scholar
  17. 17.
    Shiba, Y., Muneoka, Y., and Kanno, Y., Hiroshima J. med. Sci.27 (1978) 31.Google Scholar
  18. 18.
    Shiba, Y., and Kanno, Y., Cell Struct. Funct.14 (1989) 199.Google Scholar
  19. 19.
    Christensen, O., Nature, Lond.330 (1987) 66.Google Scholar

Copyright information

© Birkhäuser Verlag 1990

Authors and Affiliations

  • Y. Shiba
    • 1
  • Y. Kanno
    • 1
  1. 1.Department of Oral PhysiologyHiroshima University School of DentistryHiroshima(Japan)

Personalised recommendations