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Presence of specific binding sites for [3H]sarcophytol-A in cultured human skin fibroblasts

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Summary

The presence of specific binding sites for [3H]sarcophytol-A in human skin fibroblasts was examined using biochemical and morphological methods. The displacement studies clearly revealed that high (KD=31.0 nM) and low (KD=6.05 μM) affinity sites were present in the intact cells. Moreover, autoradiographic studies using light microscopy revealed that the specific binding sites may exist in boththe cytoplasm and the nuclei.

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Authors and Affiliations

  1. Group of Neuropharmacology, Josai University, 350-02, Sakado, Saitama, Japan

    T. Nakagane & R. Ishitani

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  1. T. Nakagane
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  2. R. Ishitani
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Nakagane, T., Ishitani, R. Presence of specific binding sites for [3H]sarcophytol-A in cultured human skin fibroblasts. Experientia 46, 490–492 (1990). https://doi.org/10.1007/BF01954242

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  • DOI: https://doi.org/10.1007/BF01954242

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