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Effect of phospholipids on purified lipoamidase

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Summary

Phospholipids were added to purified lipoamidase from porcine brain microsomal membranes, and changes in lipoamidase activity were examined. Approximately twofold activation of lipoamidase activity occurred upon the addition of phosphatidylethanolamine. On the other hand, phosphosphatidylserine, cardiolipin, and phosphatidic acid reduced the enzyme activity by approximately 80%. This pattern of the activation of lipoamidase by phosphatidylethanolamine and its inhibition by phosphatidylserine is similar to the pattern for adenylate cyclase, and contrasts with the pattern for ATPase.

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Authors and Affiliations

  1. Division of Metabolism, National Children's Medical Research Center, 154, Tokyo, Japan

    J. Oizumi & K. Hayakawa

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  1. J. Oizumi
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  2. K. Hayakawa
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Oizumi, J., Hayakawa, K. Effect of phospholipids on purified lipoamidase. Experientia 46, 459–461 (1990). https://doi.org/10.1007/BF01954229

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  • DOI: https://doi.org/10.1007/BF01954229

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