Pharmaceutisch Weekblad

, Volume 6, Issue 2, pp 68–74 | Cite as

Evaluation of five immunological methods for the assay of serum netilmicin

  • C. van Remmerden
  • J. R. B. J. Brouwers
  • J. A. M. Berk
  • R. J. Boskma
Original Articles


Three specific immunological methods for the determination of netilmicin and two enzyme immunoassays for gentamicin were compared for assaying serum netilmicin. The commercial kits we used were Radioimmunoassay (RIA), Substrate Labelled Fluorescence Immunoassay (gentamicin) (SLFIA), Homogeneous Enzyme Immunoassay (gentamicin and netilmicin) (EMIT) and Fluorescence Polarization Immunoassay (FPIA). After analysing spiked calf serum samples in the range of 0–15.8 mg.l−1 netilmicin, extensive statistical analysis was performed. The Performance Index was used to qualify the different methods. Results show that the SLFIA (gentamicin) kit is a very acceptable method for the determination of serum netilmicin levels, well comparable with the EMIT (gentamicin) kit and the EMIT (netilmicin) kit. FPIA and RIA require expensive specialized apparatus so it is only economically feasible when other drugs or substances are also determined on the same apparatus. The abilities and specific needs of each laboratory will play an important role in the choice of a method, because the tested kits are all within the range we need from a clinical point of view.


Gentamicin Performance Index Enzyme Immunoassay Label Fluorescence Fluorescence Polarization 


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  1. 1.
    Dahlager JI. The effect of netilmicin and other aminoglycosides on renal function, a survey of the literature on the nephrotoxicity of netilmicin. Scand J Infect Dis 1980 (suppl 23):96–102.Google Scholar
  2. 2.
    Essex-Schering Corporation. Professional Brochure, Kenilworth, New Jersey 1980:18.Google Scholar
  3. 3.
    Bäck SE, Nilsson-Ehle I, Nilsson-Ehle P. Chemical assay involving liquid chromatography for aminoglycoside antibiotics in serum. Clin Chem 1979;25:1222–5.PubMedGoogle Scholar
  4. 4.
    Broughton A, Strong JE, Pickering LK, Knight J, Bodey GP. Radioimmunoassay and Radioenzymatic assay of a new aminoglycoside antibiotic, netilmicin. Clin Chem 1978;24:717–9.PubMedGoogle Scholar
  5. 5.
    Hooymans PM, Cilissen J, Merkus FWHM. Enzyme Immunoassay of netilmicin in serum with the use of the gentamicin EMIT-kit. Am J Hosp Pharm 1982;39:784–8.PubMedGoogle Scholar
  6. 6.
    Larson T, Gerding DN, Peterson LR, Eckfeldt JH. Assay of netilmicin, using enzyme immunoassay for gentamicin. Antimicrob Agents Chemother 1982;21:399–401.PubMedGoogle Scholar
  7. 7.
    Seck J. Performance evaluation of a rapid Enzyme Immunoassay for netilmicin (Abstract). Drug Intell Clin Pharm 1982;16:481.Google Scholar
  8. 8.
    Hospes W, Boskma RJ, Brouwers JRBJ. Comparison of an HPLC method with a RIA, EMIT and FIA method for the assay of serum gentamicin with extensive statistical evaluation. Pharm Weekbl [Sci] 1982;4:32–7.Google Scholar
  9. 9.
    Wolf JA, Schwenzer KS. Automated Fluorescence Polarization Immunoassay for Monitoring Netilmicin (Abstract). Twenty-Second Interscience Conference on Antimicrobial Agents and Chemotherapy, 4–6 October 1982, Miami Beach, Florida.Google Scholar
  10. 10.
    Jolley ME, Stroupe SD, Wang C-H J, et al. Fluorescence Polarization Immunoassay 1. Monitoring aminoglycoside antibiotics in serum and plasma. Clin Chem 1981;27:1190–7.PubMedGoogle Scholar
  11. 11.
    Burd JF, Wong RC, Fenney JE, Carrico RJ, Boguslaski RC. Homogeneous reactant-labelled Fluorescent Immunoassay for therapeutic drugs exemplified by gentamicin determination in human serum. Clin Chem 1977;23:1402–8.PubMedGoogle Scholar
  12. 12.
    Burd JF, Thompson SG, Miller CA. Substrate-LabelledFluorescence Immunoassays for measuring levels of the aminoglycoside antibiotics, gentamicin, sisomicin, netilmicin, tobramycin, kanamycin and amikacin. Curr Chemother Infect Dis 1980:517–9.Google Scholar
  13. 13.
    Tallarida RJ, Murray RB. Manual of pharmacologic calculation with computer Programs. New York: Springer-Verlag, 1981.Google Scholar
  14. 14.
    Pippenger CE, Paris-Kuth H, Penry JK, Daly DD. Antiepileptic Drugs: Quantitative Analysis and Interpretation. New York: Raven Press, 1981:187–97.Google Scholar
  15. 15.
    Kaplan LA, Pesce AJ. Non-Isotopic Alternatives to Radioimmunoassay, principles and applications. New York; Marcel Dekker, Inc., 1981.Google Scholar
  16. 16.
    Barends DM, Rutgers A, Van Klingeren B, Hulshoff A. The determination of aminoglycoside antibiotics in serum: a comparison of high performance liquid chromatographic method with a microbiological assay. Pharm Weekbl [Sci] 1982;4:104–11.Google Scholar
  17. 17.
    Pesce AJ, Ditzel CA, Makler MT. Problems Associated with Enzyme Immunoassays. In: Kaplan LA, Pesce AJ, ed. Non Isotopic Alternatives to Radioimmunoassay. New York: Marcel Dekker, Inc., 1981:261.Google Scholar

Copyright information

© Royal Dutch Association for Advancement of Pharmacy 1984

Authors and Affiliations

  • C. van Remmerden
    • 1
  • J. R. B. J. Brouwers
    • 2
  • J. A. M. Berk
    • 3
  • R. J. Boskma
    • 2
  1. 1.Department of Pharmacology and PharmacotherapyState University GroningenAW GroningenThe Netherlands
  2. 2.Department of Clinical PharmacyGeneral Hospital De TjongerschansPW HeerenveenThe Netherlands
  3. 3.Department of Nuclear Medicine/In Vitro DivisionLaboratory of Clinical ChemistryEN LeeuwardenThe Netherlands

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