Evaluation of five immunological methods for the assay of serum netilmicin
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Three specific immunological methods for the determination of netilmicin and two enzyme immunoassays for gentamicin were compared for assaying serum netilmicin. The commercial kits we used were Radioimmunoassay (RIA), Substrate Labelled Fluorescence Immunoassay (gentamicin) (SLFIA), Homogeneous Enzyme Immunoassay (gentamicin and netilmicin) (EMIT) and Fluorescence Polarization Immunoassay (FPIA). After analysing spiked calf serum samples in the range of 0–15.8 mg.l−1 netilmicin, extensive statistical analysis was performed. The Performance Index was used to qualify the different methods. Results show that the SLFIA (gentamicin) kit is a very acceptable method for the determination of serum netilmicin levels, well comparable with the EMIT (gentamicin) kit and the EMIT (netilmicin) kit. FPIA and RIA require expensive specialized apparatus so it is only economically feasible when other drugs or substances are also determined on the same apparatus. The abilities and specific needs of each laboratory will play an important role in the choice of a method, because the tested kits are all within the range we need from a clinical point of view.
KeywordsGentamicin Performance Index Enzyme Immunoassay Label Fluorescence Fluorescence Polarization
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- 1.Dahlager JI. The effect of netilmicin and other aminoglycosides on renal function, a survey of the literature on the nephrotoxicity of netilmicin. Scand J Infect Dis 1980 (suppl 23):96–102.Google Scholar
- 2.Essex-Schering Corporation. Professional Brochure, Kenilworth, New Jersey 1980:18.Google Scholar
- 7.Seck J. Performance evaluation of a rapid Enzyme Immunoassay for netilmicin (Abstract). Drug Intell Clin Pharm 1982;16:481.Google Scholar
- 8.Hospes W, Boskma RJ, Brouwers JRBJ. Comparison of an HPLC method with a RIA, EMIT and FIA method for the assay of serum gentamicin with extensive statistical evaluation. Pharm Weekbl [Sci] 1982;4:32–7.Google Scholar
- 9.Wolf JA, Schwenzer KS. Automated Fluorescence Polarization Immunoassay for Monitoring Netilmicin (Abstract). Twenty-Second Interscience Conference on Antimicrobial Agents and Chemotherapy, 4–6 October 1982, Miami Beach, Florida.Google Scholar
- 12.Burd JF, Thompson SG, Miller CA. Substrate-LabelledFluorescence Immunoassays for measuring levels of the aminoglycoside antibiotics, gentamicin, sisomicin, netilmicin, tobramycin, kanamycin and amikacin. Curr Chemother Infect Dis 1980:517–9.Google Scholar
- 13.Tallarida RJ, Murray RB. Manual of pharmacologic calculation with computer Programs. New York: Springer-Verlag, 1981.Google Scholar
- 14.Pippenger CE, Paris-Kuth H, Penry JK, Daly DD. Antiepileptic Drugs: Quantitative Analysis and Interpretation. New York: Raven Press, 1981:187–97.Google Scholar
- 15.Kaplan LA, Pesce AJ. Non-Isotopic Alternatives to Radioimmunoassay, principles and applications. New York; Marcel Dekker, Inc., 1981.Google Scholar
- 16.Barends DM, Rutgers A, Van Klingeren B, Hulshoff A. The determination of aminoglycoside antibiotics in serum: a comparison of high performance liquid chromatographic method with a microbiological assay. Pharm Weekbl [Sci] 1982;4:104–11.Google Scholar
- 17.Pesce AJ, Ditzel CA, Makler MT. Problems Associated with Enzyme Immunoassays. In: Kaplan LA, Pesce AJ, ed. Non Isotopic Alternatives to Radioimmunoassay. New York: Marcel Dekker, Inc., 1981:261.Google Scholar