Experientia

, Volume 36, Issue 2, pp 253–254 | Cite as

Progesterone induced diminished incorporation of exogenous14C-amino acids in diapausing mouse blastocysts

  • Danica Dabich
  • Lon J. Van Winkle
Specialia

Summary

Under identical in vitro labeling conditions, significantly lower amounts of radioactivity were found a) in proteins of early diapausing blastocysts (days 7–9 p.c.) vs normal, late blastocysts (day 5 p.c.) and b) in proteins of days 19–23 p.c. diapausing blastocysts from ovariectomized mothers treated with progesterone vs similar embryos from untreated mothers. Thus, progesterone, which maintains the viability of embryos during prolonged diapause, causes diminished utilization of exogenous amino acids for protein synthesis in these embryos.

Keywords

Protein Synthesis Progesterone Lower Amount Label Condition Mouse Blastocyst 

References

  1. 3.
    D. R. Kirby, in: Biology of the Blastocyst. p. 393. Ed. R. J. Blandau, University of Chicago Press, Chicago 1971.Google Scholar
  2. 4.
    H. M. Weitlauf, J. Endocr.51, 375 (1971).Google Scholar
  3. 5.
    J. T. Wu, Endocrinology91, 1386 (1972).Google Scholar
  4. 6.
    T. M. Menke and A. McLaren, J. Endocr.47, 287 (1970).Google Scholar
  5. 7.
    H. M. Weitlauf, Anat. Rec.176, 121 (1973).Google Scholar
  6. 8.
    H. M. Weitlauf, J. exp. Zool.183, 303 (1973).Google Scholar
  7. 9.
    H. M. Weitlauf, J. Repord. Fert.39, 213 (1974).Google Scholar
  8. 10.
    T. M. Menke, Biol. Reprod.7, 414 (1972).Google Scholar
  9. 11.
    A. Psychoyos, Vitams Horms31, 201 (1973).Google Scholar
  10. 12.
    L. Roblero, J. Reprod. Fert.35, 153 (1973).Google Scholar
  11. 13.
    H. C. Hensleigh and H. M. Weitlauf, Biol. Repord.10, 315 (1974).Google Scholar
  12. 14.
    L. J. Van Winkle and D. Dabich, Biochem. biophys. Res. Commun.78, 357 (1977).Google Scholar
  13. 15.
    H. P. M. Pratt, J. Reprod. Fert.50, 1 (1977).Google Scholar
  14. 16.
    R. L. Brinster, Adv. Biosci.4, 199 (1970).Google Scholar
  15. 17.
    F. Bollum, Jr., in: Procedures in Nucleic Acid Research, vol. 1, p. 296, Ed. G. L. Cantoni and D. R. Davies. Harper and Row, New York 1966.Google Scholar
  16. 18.
    H. M. Weitlauf, J. exp. Zool.171, 481 (1969).Google Scholar
  17. 19.
    M. A. H. Surani, J. Cell. Sci.25, 265 (1977).Google Scholar
  18. 20.
    H. P. M. Pratt, J. Reprod. Fert.50, 1 (1977).Google Scholar
  19. 21.
    M. A. H. Surani, J. Reprod. Fert.50, 289 (1977).Google Scholar
  20. 22.
    R. J. Aitken, J. Reprod. Fert.50, 29 (1977).Google Scholar
  21. 23.
    H. M. Weitlauf, Biol. Reprod.14, 566 (1976).Google Scholar
  22. 24.
    L. J. Van Winkle, D. Dabich and T. Andary, Biol. Reprod.9, 96 (1973).Google Scholar
  23. 25.
    D. Dabich, R. A. Acey and L. D. Hazlett, in: Advances in the Study of Birth Defects, vol. 3, p. 51. Ed. T. S. N. Presaud. MTP Press Ltd, Lancaster 1979.Google Scholar
  24. 26.
    U. Westphal, in: Steroid—Protein Interaçtions, p. 434. Springer, New York 1971.Google Scholar
  25. 27.
    H. M. Weitlauf and C. S. Greenwald, J. Reprod. Fert.17, 515 (1968).Google Scholar

Copyright information

© Birkhäuser Verlag 1980

Authors and Affiliations

  • Danica Dabich
    • 1
  • Lon J. Van Winkle
    • 1
  1. 1.Department of BiochemistryWayne State University School of MedicineDetroitUSA

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