Skip to main content

Culture of presumptive epithelial cells from jejunal mucosa of axenic rats

Summary

Strains of presumptive epithelial and fibroblast cells were prepared from the jejunal mucosa of axenic rats. Cells were cultured on collagen gels, in highly enriched media supplemented with homologous sera and hormones, and were maintained for more than 7 weeks.

This is a preview of subscription content, access via your institution.

References

  1. G. Henle and F. Deinhardt, J. Immun.79, 54 (1957).

    Google Scholar 

  2. L. Lichtenberger, L.R. Miller, D.N. Erwin and L.R. Johnson, Gastroenterology65, 242 (1973).

    Google Scholar 

  3. C.F.A. Culling, P.E. Reid, L.S. Trueman and W.L. Dunn, Proc. Soc. exp. Biol. Med.142, 434 (1973).

    Google Scholar 

  4. A.G.D. Hoffman and A. Kuksis, Gastroenterology72, 1160a (1977).

    Google Scholar 

  5. C.M. Schiller, In Vitro13, 198 (1977).

    Google Scholar 

  6. F. Raul, M. Kedinger, P. Simon, J. Grenier and K. Haffen, Biol. cell.33, 163 (1978).

    Google Scholar 

  7. P. Pothier and J.S. Hugon, J. Cell Biol.79, 86a (1978).

    Google Scholar 

  8. A.G.D. Hoffman and A. Kuksis, manuscript submitted.

  9. R.P. Spencer, The Intestinal Tract. Ryerson Press, Toronto 1960.

    Google Scholar 

  10. J.T. Emerson and D.R. Pitelka, In Vitro13, 316 (1977).

    Google Scholar 

  11. R.G. Ham, in: Tissue Culture—Methods and Applications, section V, chapter 3. Ed. P.F. Kruse, Jr, and M.R. Patterson, Jr. Academic Press, New York 1973.

    Google Scholar 

  12. S.F. Gilbert and B.R. Migeon, Cell5, 11 (1975).

    Google Scholar 

  13. D. Perlman, in: Cell Culture, section 7. Methods in Enzymology, vol. 58. Ed. W.B. Jakoby and I.H. Pastan. Academic Press, New York 1979.

    Google Scholar 

  14. L.R. Johnson, Gastroenterology72, 788 (1977).

    Google Scholar 

  15. T.J. Peters and H. Shio, Clin. Sci. molec. Med.50, 355 (1976).

    Google Scholar 

  16. A.M. Carriere, Anat. Rec.156, 423 (1966).

    Google Scholar 

  17. H. Green, Cell11, 405 (1977).

    Google Scholar 

  18. A. Quaroni, J. Wands, R. Trelstad and K.J. Isselbacher, J. Cell Biol.80, 248 (1979).

    Google Scholar 

Download references

Author information

Affiliations

Authors

Additional information

Supported by the Medical Research Council (Canada). The authors gratefully acknowledge the advice of Dr P. Rae of this institute.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Hoffman, A.G.D., Kuksis, A. Culture of presumptive epithelial cells from jejunal mucosa of axenic rats. Experientia 36, 202–204 (1980). https://doi.org/10.1007/BF01953732

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01953732

Keywords

  • Collagen
  • Epithelial Cell
  • Fibroblast Cell
  • Enrich Medium
  • Jejunal Mucosa