Experientia

, Volume 38, Issue 8, pp 898–899 | Cite as

Identification of (3R,4R)-gD1(6) as an isolation artefact of cannabinoid acids formed by callus cultures ofCannabis sativa L

  • A. Heitrich
  • M. Binder
Specialia Chemica, Biochimica

Summary

For the first time we have isolated a major psychoactive cannabinoid, (3R, 4R)-Δ1(6)-tetrahydrocannabinol3 from callus cultures ofCannabis sativa L.3 was obtained as an artefact of the actually formed (3R, 4R)-Δ1-tetrahydrocannabinol-3′- and/or 5′-carboxylic acids1 and2 by subjecting the culture material to a decarboxylation step prior to extraction. No attempt was made to isolated acids1 and2. The identity of3 was confirmed by comparison with an authentic sample of (3R, 4R)-Δ1-tetrahydrocannabinol. Culture conditions, isolation procedure and identification of the cannabinoid are described.

Keywords

Culture Condition Carboxylic Acids1 Callus Culture Authentic Sample Culture Material 

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Copyright information

© Birkhäuser Verlag 1982

Authors and Affiliations

  • A. Heitrich
    • 1
  • M. Binder
    • 1
  1. 1.Institut für Physiologische ChemieRuhr-Universität BochumBochum 1Germany

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