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Electron probe x-ray microanalysis and cryoultramicrotomy of unstained myocardial sarcoplasmic reticulum, in situ and fragmented

Experientia volume 35, pages 1591–1593 (1979)Cite this article

Summary

Myocardial sarcoplasmic reticulum of cats in situ and fragmented sarcoplasmic reticulum (FSR) were analysed using X-ray microanalysis, cryoultramicrotomy and scanning transmission electron microscopy. 2 types of FSR vesicles can be distinguished morphologically and by their different elemental composition especially by different Ca loading. The Ca content of the sarcoplasmic reticulum can also be detected in situ.

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Author information

Affiliations

  1. Institute of Physiology II, University of Tübingen, Gmelinstrasse 5, D-7400, Tübingen, (Federal Republic of Germany)

    M. F. Wendt-Gallitelli, H. Wolburg, M. Schwegler & W. Schlore

  2. Institute of Submicroscopic Pathology, University of Tübingen, Liebermeisterstrasse 8, D-7400, Tübingen, (Federal Republic of Germany)

    M. F. Wendt-Gallitelli, H. Wolburg, M. Schwegler & W. Schlore

Authors
  1. M. F. Wendt-Gallitelli
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  2. H. Wolburg
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  3. M. Schwegler
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  4. W. Schlore
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Additional information

Acknowledgments. We thank Drs A.V. Somlyo and A.P. Somlyo for helpful advice and valuable discussion.

This work was supported by the Deutsche Forschungsgemeinschaft.

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Wendt-Gallitelli, M.F., Wolburg, H., Schwegler, M. et al. Electron probe x-ray microanalysis and cryoultramicrotomy of unstained myocardial sarcoplasmic reticulum, in situ and fragmented. Experientia 35, 1591–1593 (1979). https://doi.org/10.1007/BF01953211

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  • DOI: https://doi.org/10.1007/BF01953211

Keywords

  • Microscopy
  • Electron Microscopy
  • Transmission Electron Microscopy
  • Elemental Composition
  • Sarcoplasmic Reticulum