Experientia

, Volume 50, Issue 7, pp 677–679 | Cite as

Microdialysis study of ischemia-induced hydroxyl radicals in the canine heart

  • A. A. Timoshin
  • O. V. Tskitishvili
  • L. I. Serebryakova
  • A. I. Kuzmin
  • O. S. Medvedev
  • E. K. Ruuge
Research Articles

Abstract

A new experimental approach for spin-trapping of oxygen radicals in a selected region of the heart in situ is described. This approach is based on microdialysis, and it permits the detection of oxygen radicals in conditions of local ischemia and restoration of normal blood flow. Increased hydroxyl radical generation in an ischemic area of canine myocardium, as a result of 40 min local occlusion, has been studied.

Key words

Canine heart electron paramagnetic resonance local ischemia microdialysis oxygen radicals spintrapping 

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Copyright information

© Birkhäuser Verlag 1994

Authors and Affiliations

  • A. A. Timoshin
    • 1
  • O. V. Tskitishvili
    • 1
  • L. I. Serebryakova
    • 1
  • A. I. Kuzmin
    • 1
  • O. S. Medvedev
    • 1
  • E. K. Ruuge
    • 1
  1. 1.Institute of Experimental Cardiology, Cardiology Research CenterRussian Academy of Medical SciencesMoscow(Russia)

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