Experientia

, Volume 38, Issue 4, pp 426–427 | Cite as

Inhibition of cytosolic rat hepatic glutathione S-transferase activities by bromosulfophthaleins

  • A. J. Baars
  • M. Jansen
  • S. Arnoldussen
  • K. L. de Lussanet de la Sablonière
  • D. D. Breimer
Specialia

Summary

The enzymatically catalyzed conjugation of glutathione with 1-chloro-2,4-dinitrobenzene and styrene-7,8-oxide is inhibited by tetrabromosulfophthalein in a non-competetive way, although tetrabromosulfophthalein itself is a substrate for glutathione S-transferases. Dibromosulfophthalein, which is not a substrate for glutathione S-transferases, inhibits the 1-chloro-2,4-dinitrobenzene conjugation competetively and the styrene-7,8-oxide conjugation non-competetively.

Keywords

Oxide Glutathione Dinitrobenzene Hepatic Glutathione Oxide Conjugation 

Literature

  1. 1.
    W.B. Jakoby, Adv. Enzym.46, 383 (1978).Google Scholar
  2. 2.
    P. Askelöf, C. Guthenberg, I. Jakobsson and B. Mannervik, Biochem. J.147, 513 (1975).PubMedGoogle Scholar
  3. 3.
    W.B. Jakoby, J.N. Ketley and W.H. Habig, in: Glutathione: metabolism and function, p. 213. Eds I.M. Arias and W.B. Jakoby. Raven Press, New York 1976.Google Scholar
  4. 4.
    W.B. Jakoby, W.H. Habig, J.H. Keen, J.N. Ketley and M.J. Pabst, in: Glutathione: metabolism and function, p. 189. Eds I.M. Arias and W.B. Jakoby. Raven Press, New York 1976.Google Scholar
  5. 5.
    A.J. Baars, M. Jansen and D.D. Breimer, Biochem. Pharmac.27, 2487 (1978).CrossRefGoogle Scholar
  6. 6.
    J. Marniemi and M.G. Parkki, Biochem. Pharmac.24, 1569 (1975).CrossRefGoogle Scholar
  7. 7.
    D.K.F. Meijer, R.J. Vonk, K. Keulemans and J.G. Weitering, J. Pharmac. exp. Ther.207, 8 (1977).Google Scholar

Copyright information

© Birkhäuser Verlag 1982

Authors and Affiliations

  • A. J. Baars
    • 1
  • M. Jansen
    • 1
  • S. Arnoldussen
    • 1
  • K. L. de Lussanet de la Sablonière
    • 1
  • D. D. Breimer
    • 1
  1. 1.Department of Pharmacology, Subfaculty of Pharmacy, Sylvius LaboratoriesUniversity of LeidenLeiden(The Netherlands)

Personalised recommendations