, Volume 49, Issue 10, pp 906–909 | Cite as

PCR jumping in clones of 30-million-year-old DNA fragments from amber preserved termites (Mastotermes electrodominicus)

  • R. DeSalle
  • M. Barcia
  • C. Wray
Research Articles


DNA from 30-million-year-old amber preserved termites (Mastotermes electrodominicus) was PCR amplified with nuclear ribosomal RNA small subunit primers and cloned into the TA vector (INVITROGEN). We obtained several classes of recombinant clones as a result. AuthenticMastotermes electrodominicus clones were identified. The source of other classes of clones was identified as contaminants of the ancient DNA template. Several of the clones appeared to be chimeric in structure with half of the clone identical to the termite sequence and the other half identical to contaminant sequences. The phenomenon of PCR jumping was identified as a possible source for the chimeric clones.

Key words

Polymerase chain reaction ancient DNA termite relationships Mastotermes electrodominicus 


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Copyright information

© Birkhäuser Verlag 1993

Authors and Affiliations

  • R. DeSalle
    • 1
  • M. Barcia
    • 1
  • C. Wray
    • 1
    • 2
  1. 1.Department of EntomologyAmerican Museum of Natural HistoryNew YorkUSA
  2. 2.Department of Geology and GeophysicsYale UniversityNew HavenUSA

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