PCR jumping in clones of 30-million-year-old DNA fragments from amber preserved termites (Mastotermes electrodominicus)
- 135 Downloads
DNA from 30-million-year-old amber preserved termites (Mastotermes electrodominicus) was PCR amplified with nuclear ribosomal RNA small subunit primers and cloned into the TA vector (INVITROGEN). We obtained several classes of recombinant clones as a result. AuthenticMastotermes electrodominicus clones were identified. The source of other classes of clones was identified as contaminants of the ancient DNA template. Several of the clones appeared to be chimeric in structure with half of the clone identical to the termite sequence and the other half identical to contaminant sequences. The phenomenon of PCR jumping was identified as a possible source for the chimeric clones.
Key wordsPolymerase chain reaction ancient DNA termite relationships Mastotermes electrodominicus
Unable to display preview. Download preview PDF.
- 7.Patton, J. L., and Smith, M. F., Evolution46 (1992) 148.Google Scholar
- 10.Yokoi, T., Aoki, Y., and Sagisaki, K., Zs. Rechtsmedizin102 (1989) 323.Google Scholar
- 11.Williams, S. R., Longmire, J. L., and Beck, L. A., Am. J. Anthropol.812 (1990) 318.Google Scholar
- 14.Cano, R. J., Poinar, H., and Poinar, G. O., Med. Sci. Res.20 (1992) 249.Google Scholar
- 15.Cano, R. J., Poinar, H., and Poinar, G. O., Med. Sci. Res.20 (1992) 619.Google Scholar
- 18.Maniatis, T., Fritsch, E. F., and Sambrook, J., Molecular Cloning: A Laboratory Manual. Cold Spring Harbour Press, Cold Spring Harbour, New York 1982.Google Scholar
- 19.Swofford, D. L., Phylogenetic Analysis Using Parsimony (PAUP). Illinois Natural History Survey, 1990.Google Scholar
- 20.Higuchi, R., and Wilson, A. C., Fedn Proc.43 (1984) 1557.Google Scholar
- 22.Bradley, R. D., Bull, J. J., and Hillis, D. M., personal communication, 1992.Google Scholar