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Makisterone A and 24-methylenecholesterol from the ovaries of the honey bee,Apis mellifera L

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Summary

Makisterone A, a 28-carbon ecdysteroid (molting hormone) has been isolated from the ovaries of queen bees. Analysis by reversed-phase and silica high performance liquid chromatography (HPLC) in conjunction with a radioimmune assay (RIA) revealed about 11 ng of makisterone A present per gram of ovaries on a fresh weight basis. No C27 ecdysteroids were detected. The predominant neutral sterol present was 24-methylenecholesterol.

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Authors and Affiliations

  1. Insect Physiology Laboratory, ARS, USDA, 20705, Beltsville, Maryland, USA

    M. F. Feldlaufer & J. A. Svoboda

  2. Bioenvironmental Bee Laboratory, ARS, USDA, 20705, Beltsville, Maryland, USA

    E. W. Herbert Jr

Authors
  1. M. F. Feldlaufer
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  2. J. A. Svoboda
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  3. E. W. Herbert Jr
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Feldlaufer, M.F., Svoboda, J.A. & Herbert, E.W. Makisterone A and 24-methylenecholesterol from the ovaries of the honey bee,Apis mellifera L. Experientia 42, 200–201 (1986). https://doi.org/10.1007/BF01952468

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  • DOI: https://doi.org/10.1007/BF01952468

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