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A thiol protease of peritoneal macrophages in the guinea pig

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  • Biochemistry and Biophysics, Metabolism, Neurobiology, Pharmacology
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Summary

Proteolytic enzymes of the guinea pig peritoneal exudate macrophages were investigated using synthetic fluorogenic peptide substrates. Among several enzymes, t-butyloxycarbonyl-phenylalanyl-seryl-arginine 4-methylcoumaryl-7-amide cleaving enzymes had the highest activity, and the activity in exudate macrophages was about 3 times tronger than that in resident thiol-blocking reagents, suggesting a thiol protease.

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Authors and Affiliations

  1. Department of Allergy, Institute for Medical Immunology, Kumamoto University Medical School, Honjo 2-2-1, 860, Kumamoto, Japan

    T. Kambara, S. Uchida, J. Tanaka & S. Shoji

  2. Department of Biochemistry, Faculty of Pharmaceutical Sciences, Kumamoto University, 862, Kumamoto, Japan

    T. Kambara, S. Uchida, J. Tanaka & S. Shoji

Authors
  1. T. Kambara
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  2. S. Uchida
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  3. J. Tanaka
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  4. S. Shoji
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Additional information

We thank Dr Yoshiaki Motozato, Kumamoto University, for kind donation of Cellulofine GC-700 and valuable discussions. This work is supported in part by the Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.

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Kambara, T., Uchida, S., Tanaka, J. et al. A thiol protease of peritoneal macrophages in the guinea pig. Experientia 42, 155–157 (1986). https://doi.org/10.1007/BF01952443

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  • DOI: https://doi.org/10.1007/BF01952443

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