, Volume 40, Issue 5, pp 483–485 | Cite as

Colony hybridization method for screening in situ of eukaryotic amplified genes

  • Y. Malpièce
  • M. L. Michel
  • G. Carloni


A new method for autoradiographic screening of amplified genes in cellular clones is described. The main feature of the device is to keep viable cells from each clone, which can subsequently be regrown. The availability of this biochemical screening method allows screening for recombinants harboring unselectable markers as well.


Viable Cell Screening Method Hybridization Method Colony Hybridization Biochemical Screening 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Benton, W. D., and Davis, R. W., Science196 (1977) 180.PubMedGoogle Scholar
  2. 2.
    Mulligan, R. C., and Berg, P., Science209 (1980) 1422.PubMedGoogle Scholar
  3. 3.
    Mulligan, R. C., and Berg, P., Molec. cell. Biol.1 (1981) 449.PubMedGoogle Scholar
  4. 4.
    Berg, P., Science213 (1981) 296.PubMedGoogle Scholar
  5. 5.
    Wigler, M., Pellicer, A., Silverstein, S., Axel, R., Urlaub, G., and Chasin, L., Proc. natl Acad. Sci. USA78 (1979) 3567.Google Scholar
  6. 6.
    Wigler, M., Perrucho, M., Kurtz, D., Dana, S., Pellicer, A., Axel, R., and Silverstein, S., Proc. natl Acad. Sci. USA77 (1980) 3567.PubMedGoogle Scholar
  7. 7.
    Pourcel, C., Louise, A., Gervais, M., Chenciner, N., Dubois, M. F., and Tiollais, P., J. Virol.42 (1982) 100.PubMedGoogle Scholar
  8. 8.
    Chang, A. C. Y., Numberg, G. H., Kaufman, R. J., Erlich, H. A., Schimke, R. T., and Cohen, S. N., Nature279 (1978) 617.Google Scholar
  9. 9.
    Dubois, M. F., Pourcel, C., Rousset, S., Chany, C., and Tiollais, P., Proc. natl Acad. Sci. USA77 (1980) 4549.PubMedGoogle Scholar
  10. 10.
    Hanahan, D., and Meselson, M., Gene10 (1980) 63.CrossRefPubMedGoogle Scholar
  11. 11.
    Grunstein, M., and Hogness, D. S., Proc. natl Acad. Sci. USA72 (1975) 3961.PubMedGoogle Scholar
  12. 12.
    Cami, B., and Kourilsky, P., Nucleic Acids Res.5 (1978) 2381.PubMedGoogle Scholar
  13. 13.
    Denhardt, D. T., Biochem. biophys. Res. Commun.23 (1966) 641.CrossRefPubMedGoogle Scholar
  14. 14.
    Rigby, P. J. W., Dieckmann, M., Rhodes, C., and Berg, P., J. molec. Biol.113 (1977) 237.PubMedGoogle Scholar
  15. 15.
    Southern, E., J. molec. Biol.98 (1975) 503.PubMedGoogle Scholar
  16. 16.
    Chenciner, N., Meneguzzi, G., Corallini, A., Grossi, M. P., Grassi, P., Barbanti-Brodano, G. and Milanesi, G., Proc. natl Acad. Sci. USA77 (1980) 975.PubMedGoogle Scholar
  17. 17.
    Galibert, F., Mandart, E., Fitoussi, F., Tiollais, P., and Charnay, P., Nature281 (1979) 646.PubMedGoogle Scholar
  18. 18.
    Wetmur, J. G., Biopolymers14 (1975) 2517.CrossRefGoogle Scholar
  19. 19.
    Gerhardt, D. S., Kawasaki, E. S., Bancroft, F. C., and Szabo, P., Proc. natl Acad. Sci. USA78 (1981) 3755.PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1984

Authors and Affiliations

  • Y. Malpièce
    • 1
    • 2
  • M. L. Michel
    • 1
    • 2
  • G. Carloni
    • 1
    • 2
  1. 1.Groupement de Génie GénétiqueInstitut PasteurParis Cedex 15(France)
  2. 2.Centro Virus RespiratoriCNR, Policlinico Umberto IRoma(Italy)

Personalised recommendations