Experientia

, Volume 44, Issue 2, pp 142–145 | Cite as

Human platelet 5-hydroxytryptamine receptors: Binding of [3H]-lysergic acid diethylamide (LSD). Effects of chronic neuroleptic and antidepressant drug administration

  • D. G. Grahame-Smith
  • D. P. Geaney
  • M. Schachter
  • J. M. Elliott
Multi-author Review The Platelet in Pathophysiological Research

Summary

Chronic treatment with phenothiazines and thioxanthenes has been found to enhance 5-HT-induced aggregation of human platelets. A method has been developed to study 5-HT2 receptor binding sites on platelets utilising [3H]-LSD and more recently125I/LSD. Results are presented which suggest that the LSD binding site is indeed the 5-HT2 binding site and that the LSD binding characterises the specific receptor responsible for 5-HT-induced shape change and aggregation.

In a group of patients receiving phenothiazines or thioxanthenes, theBmax of LSD binding was increased. The mean binding affinity was decreased possibly due to a persistence of neuroleptic in the platelet membrane preparation. Analysis showed that this was not the reason why the mean binding capacity was increased.

The results show that chronic phenothiazine and thioxanthene δ treatment ‘up-regulates’ platelet 5-HT2 binding sites and that this may be accompanied by increased sensitivity to platelet aggregation by 5-HT.

In normal subjects desipramine treatment increased theBmax of platelet LSD binding and this was accompanied by an increased prolactin response to tryptophan which is thought to be mediated by central 5-HT function.

Key words

Platelets 5-HT LSD binding neuroleptics 

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Copyright information

© Birkhäuser Verlag 1988

Authors and Affiliations

  • D. G. Grahame-Smith
    • 1
  • D. P. Geaney
    • 1
  • M. Schachter
    • 1
  • J. M. Elliott
    • 1
  1. 1.MRC Unit and University Department of Clinical PharmacologyRadcliffe InfirmaryOxford(England)

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