, Volume 44, Issue 2, pp 97–100 | Cite as

Cytosolic calcium in platelet activation

  • T. J. Rink
Multi-author Review The Platelet in Pathophysiological Research


Experiments with permeabilised platelets, and with intact platelets loaded with fluorescent Ca2+-indicators, over the past several years have greatly extended our knowledge and understanding of cytosolic Ca2+ as a platelet activator and its interactions with other cytosolic regulators. This article outlines insights, gained from the use of the fluorescent dyes, into maintenance and restoration of basal [Ca2+]i, mechanisms of receptor-mediated Ca2+-mobilisation and quantitation of [Ca2+]i/response relations in intact human platelets.

Key words

Calcium platelet second messenger 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Brass, L.F., Laposata, M., Banga, H.S., and Rittenhouse, S.E., Regulation of the phosphoinositide hydrolysis pathway in thrombinstimulated platelets by a pertussis toxin-sensitive guanine nucleotidebinding protein. J. biol. Chem.261 (1986) 16838–16847.PubMedGoogle Scholar
  2. 2.
    Enyedi, A., Sarkadi, B., Foldes-Papp, Z., Monostory, S., and Gardos, G., Demonstration of two distinct calcium pumps in human platelet membrane vesicles. J. biol. Chem.261 (1986) 9558–9563.PubMedGoogle Scholar
  3. 3.
    Feinstein, M.B., and Fraser, C., Human platelet secretion and aggregation induced by calcium ionophores. J. gen. Physiol.66 (1975) 561–581.PubMedGoogle Scholar
  4. 4.
    Hack, N., Croset, M., and Crawford, N., Studies on the bivalentcation activated ATPase activities of highly purified human platelet surface and intracellular membranes. Biochem. J.233 (1986) 661–668.PubMedGoogle Scholar
  5. 5.
    Hallam, T.J., Daniel, J.L., Kendrick-Jones, J., and Rink, T.J., Relationship between cytoplasmic free calcium and myosin light chain phosphorylation in intact platelets. Biochem. J.232 (1985) 373–377.PubMedGoogle Scholar
  6. 6.
    Hallam, T.J., and Rink, T.J., Response to adenosine diphosphate in human platelets loaded with the fluorescent Ca2+ indicator quin 2. J. Physiol.368 (1985) 131–146.PubMedGoogle Scholar
  7. 7.
    Hallam, T.J., and Rink, T.J., Agonists stimulate divalent cation channels in the plasma membrane of human platelets. FEBS Lett.186 (1985) 175–179.PubMedGoogle Scholar
  8. 8.
    Hallam, T.J., Sanchez, A., and Rink, T.J., Stimulus-response coupling in human platelets: changes evoked by platelet-activation factor in cytoplasmic free calcium monitored with the fluorescent Ca indicator, quin 2. Biochem. J.218 (1984) 819–827.PubMedGoogle Scholar
  9. 9.
    Knight, D.E., and Scrutton, M.C., Direct evidence for a role for Ca2+ in amine storage granule secretion by human platelets. Thrombosis Res.20 (1980) 437–446.Google Scholar
  10. 10.
    Knight, D.E., and Scrutton, M.C., Gaining access to the cytosol: the technique and applications of electropermeabilization. Biochem. J.234 (1986) 497–506.PubMedGoogle Scholar
  11. 11.
    MacIntyre, D.E., Buchfield, M., and Shaw, A.M., Regulation of platelet cytosolic free calcium by cyclic nucleotides and protein kinase C. FEBS Lett.188 (1985) 383–388.PubMedGoogle Scholar
  12. 12.
    Massini, P., and Luscher, E.F., Some effects of ionophores for divalent cations on blood platelets. Comparison with the effects of thrombin. Biochim. biophys. Acta372 (1974) 109–121.PubMedGoogle Scholar
  13. 13.
    Massini, P., and Luscher, E.F., On the significance of the influx of calcium ions into stimulated human blood platelets. Biochim. biophys. Acta416 (1976) 652–663.Google Scholar
  14. 14.
    O'Rourke, F.A., Halenda, S.P., Zavoico, G.B., and Feinstein, M.B., Inositol trisphosphate-induced Ca2+ release from human platelet membranes. J. biol. Chem.260 (1985) 956–962.PubMedGoogle Scholar
  15. 15.
    Pipili, E., Platelet membrane potential: simultaneous measurement of dis-C3-(5) fluorescence and optical density. Thromb. Haemostasis54 (1985) 645–651.Google Scholar
  16. 16.
    Pollock, W.K., Irvine, R.F., and Rink, T.J., Free Ca2+ requirements of agonist-induced thromboxane-A2 synthesis in human platelets. Eur. J. Pharmac.132 (1986) 309–313.Google Scholar
  17. 17.
    Pollock, W.K., and Rink, T.J., Thrombin and ionomycin can raise platelet cytosolic Ca2+ to micromolar levels by discharge of internal Ca2+ stores: studies using fura-2. Biochem. biophys. Res. Commun.139 (1986) 308–314.PubMedGoogle Scholar
  18. 18.
    Pollock, W.K., Rink, T.J., and Irvine, R.F., Liberation of [3H] arachidonic acid and changes in cytosolic free calcium in fura-2-loaded human platelets stimulated by ionomycin and collagen. Biochem. J.235 (1986) 869–877.PubMedGoogle Scholar
  19. 19.
    Pollock, W.K., Sage, S.O., and Rink, T.J., Stimulation of Ca2+ efflux from fura-2-loaded platelets activated by thrombin or phorbol myristate acetate. FEBS Lett.210 (1987) 132–136.PubMedGoogle Scholar
  20. 20.
    Rink, T.J., and Hallam, T.J., What turns platelets on? Trends biochem. Sci.9 (1984) 215–219.Google Scholar
  21. 21.
    Rink, T.J., Sanchez, A., and Hallam, T.J., Diacylglycerol and phorbol ester stimulate secretion without raising cytoplasmic free calcium in human platelets. Nature305 (1983) 317–319.PubMedGoogle Scholar
  22. 22.
    Rink, T.J., Smith, S.W., and Tsien, R.Y., Cytoplasmic free Ca2+ in human platelets: Ca2+ thresholds and Ca-independent activation for shape-change and secretion. FEBS Lett.148 (1982) 21–26.PubMedGoogle Scholar
  23. 23.
    Sage, S.O., and Rink, T.J., Inhibition by forskolin of cytosolic calcium rise, shape change and aggregation in quin 2-loaded human platelets. FEBS Lett.188 (1985) 135–140.PubMedGoogle Scholar
  24. 24.
    Sage, S.O., and Rink, T.J., Effects of ionic substitution on [Ca2+]i rises evoked by thrombin and PAF in human platelets. Eur. J. Pharmac.128 (1986) 99–107.Google Scholar
  25. 25.
    Sage, S.O., and Rink, T.J., Kinetic differences between thrombin-induced and ADP-induced calcium influx and release from internal stores in fura-2-loaded human platelets. Biochem. biophys. Res. Commun.136 (1986) 1124–1129.PubMedGoogle Scholar
  26. 26.
    Simpson, A.W.M., Hallam, T.J., and Rink, T.J., Low concentrations of the stable prostaglandin endoperoxide U 44 069 stimulate shape change in quin2-loaded platelets without a measurable increase in [Ca2+]i. FEBS Lett.201 (1986) 301–305.PubMedGoogle Scholar
  27. 27.
    Steiner, B., and Luscher, E.F., Evidence that the platelet plasma membrane does not contain a [Ca2++Mg2+]-dependent ATPase. Biochem. biophys. Acta818 (1985) 299–309.PubMedGoogle Scholar
  28. 28.
    Tsien, R.Y., Pozzan, T., and Rink, T.J., Calcium homeostasis intact lymphocytes: cytoplasmic free calcium monitored with a new, intracellularly trapped fluorescent indicator. J. Cell Biol.94 (1982) 335–340.PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1988

Authors and Affiliations

  • T. J. Rink
    • 1
  1. 1.Smith Kline & French Research LtdWelwyn(England)

Personalised recommendations