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In vitro systems in the study of peroxisomal protein import

Experientia volume 52, pages 1055–1062 (1996)Cite this article

Abstract

Our level of understanding of peroxisome biogenesis in comparison with other cellular organelles is rudimentary, yet the fragments of information available indicate that the targeting and import of peroxisomal proteins occur by fundamentally different mechanisms. Genetic studies have identified a number of genes required for peroxisome assembly, but in most cases the functions of the gene products remain unknown. In vitro protein translocation systems have played a prominent role in unravelling the biochemistry of protein translocation into other organelles. This review considers some of the requirements for establishing a bona fide peroxisomal import assay and discusses the findings which have emerged as a result of using such experimental systems.

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Affiliations

  1. Centre for Plant Biochemistry and Biotechnology, University of Leeds, LS2 9JT, Leeds, UK

    A. Baker

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Baker, A. In vitro systems in the study of peroxisomal protein import. Experientia 52, 1055–1062 (1996). https://doi.org/10.1007/BF01952102

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  • DOI: https://doi.org/10.1007/BF01952102

Key words

  • Peroxisomes
  • glyoxysomes
  • in vitro protein translocation assay
  • targeting signals
  • organelle biogenesis