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Molecular machinery mediating vesicle budding, docking and fusion

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Abstract

A general machinery buds and fuses transport vesicles which connect intracellular compartments with each other and allow communication with the extracellular environment. Cytoplasmic coat proteins deform membranes to bud vesicles and interact directly or indirectly with cargo molecules. Compartment-specific SNAREs (SNAP receptors) on vesicles and target membranes dock vesicles and provide a scaffolding for the general fusion machinery to initiate lipid bilayer fusion.

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Abbreviations

NSF:

N-ethylmaleimide-sensitive fusion protein

SNAP:

soluble NSF attachment protein

SNARE:

SNAP receptor

ER:

endoplasmic reticulum

ARF:

ADP-ribosylation factor

NEM:

N-ethylmaleimide

GAP:

GTPase activating protein

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  1. Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, Box 251, 10021, New York, New York, USA

    T. H. Söllner & J. E. Rothman

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Söllner, T.H., Rothman, J.E. Molecular machinery mediating vesicle budding, docking and fusion. Experientia 52, 1021–1025 (1996). https://doi.org/10.1007/BF01952097

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