Summary
Monoclonal antibodies to a surface antigen of the modulated smooth muscle cells originally isolated from the rat aorta media were conjugated with ricin A-chain via an oxidized dextran bridge. The interaction of cultured cells with the conjugates obtained and with control substances was monitored following incorporation of14C-leucine radioactivity. It was found that14C-leucine incorporation was suppressed by 80–90% at a conjugate concentration of 10−6–10−7 M. Antigen-negative cells (line IAR; rat hepatocytes) were insensitive to the conjugate at any concentration used. Control use of purified ricin A-chain, native or oxidized dextran, specific and nonspecific IgG did not affect normal14C-leucine incorporation. The data obtained may be useful for designing targeted drug transport systems and for selective screening of modulated smooth cells in vascular pathology models in vivo.
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Printseva, O.Y., Faerman, A.I., Maksimenko, A.V. et al. Selective killing of smooth muscle cells in culture by the ricin A-chain conjugated with monoclonal antibodies to a cell surface antigen via a dextran bridge. Experientia 41, 1342–1344 (1985). https://doi.org/10.1007/BF01952086
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DOI: https://doi.org/10.1007/BF01952086