Summary
A very simple fluorometric assay for polyamine oxidase (PAO) in tissues, with N1-monoacetylspermine as substrate, is described. The PAO was present in all human organs tested; it was highest in the liver, followed by the testis, kidney, spleen and small intestine.
Literatur
Hölttä, E., Biochemistry16 (1977) 91.
Seiler, N., Bolkenius, F. N., Knödgen, B., and Mamont, P., Biochim. biophys. Acta615 (1980) 480.
Bolkenius, F. N., and Seiler, N., Int. J. Biochem.13 (1981) 287.
Seiler, N., and Knödgen, B., J. Chromat.164 (1979) 155.
Tabor, H., Tabor, C. W., and de Meis, L., in: Methods in Enzymology, vol. 17, part B, p. 829. Eds H. Tabor and C. W. Tabor. Academic Press, New York 1971.
Guilbault, G. G., Brignac, P. J. Jr, and Juneau, M., Analyt. Chem.40 (1968) 1256.
Snyder, S. H., and Hendley, E. D., J. Pharmac. exp. Ther163 (1968) 386..
Matsumoto, T., Furuta, T., Nimura, Y., and Suzuki, O., Biochem. Pharmac.31 (1982) 2207.
Suzuki, O., Matsumoto, T., Oya, M., and Katsumata, Y., Biochim. biophys. Acta677 (1981) 190.
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Suzuki, O., Matsumoto, T. & Katsumata, Y. Determination of polyamine oxidase activities in human tissues. Experientia 40, 838–839 (1984). https://doi.org/10.1007/BF01951981
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DOI: https://doi.org/10.1007/BF01951981