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K-p-Nitrophenylphosphatase activity, Na and K content, Na permeability and membrane lipid composition in rabbit myocardium after cholesterol rich diet

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The aim of the present study was to investigate the effects of a cholesterol-rich diet on membrane function and lipid composition in rabbit myocardium. The activity and the ouabain sensitivity of the K-p-nitrophenylphosphatase (K-pNPPase), a partial reaction of the Na, K-ATPase, were diminished after a cholesterol/oil or pure cholesterol diet. The content of cholesterol, cholesterol esters and of several classes of phospholipids was enhanced in microsomes. A causal relationship is assumed between cholesterol accumulation and a decrease in membrane fluidity as well as in Na, K-ATPase activity. The intracellular Na content and the Na-Li-exchange rate were higher after the cholesterol diet. The increase in the Na content is supposed to be induced by a lower Na transport and a higher Na permeability. An enhanced Ca flux via the sarcolemma could be the consequence.

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Authors and Affiliations

  1. Institute of Physiology, Institute of pathological and clinical Biochemistry, Humboldt University of Berlin, DDR-1040, Berlin, German Democratic Republic

    E. Kutscherskij, J. Günther & E. Mehley

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  1. E. Kutscherskij
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  2. J. Günther
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  3. E. Mehley
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Kutscherskij, E., Günther, J. & Mehley, E. K-p-Nitrophenylphosphatase activity, Na and K content, Na permeability and membrane lipid composition in rabbit myocardium after cholesterol rich diet. Experientia 40, 812–815 (1984). https://doi.org/10.1007/BF01951966

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