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A simplified method for isocratic HPLC analysis of polyamines

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Summary

A simple technique is described for the separation and analysis of polyamines in tissues and body fluids, utilizing precolumn clean-up on disposable CM-cellulose columns, followed by an automatable HPLC procedure. Complete separation and analysis takes 12–15 min per sample with sensitivity in the pmole range.

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Authors and Affiliations

  1. Department of Pharmacology, Duke University Medical Center, 27710, Durham, North Carolina, USA

    W. L. Whitmore & T. A. Slotkin

Authors
  1. W. L. Whitmore
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  2. T. A. Slotkin
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Additional information

Acknowledgments. Supported by USPHS HD-09713, EPA R811621010 and by Research Scientist Development Award DA-00006 to TAS.

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Whitmore, W.L., Slotkin, T.A. A simplified method for isocratic HPLC analysis of polyamines. Experientia 41, 1209–1211 (1985). https://doi.org/10.1007/BF01951733

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  • DOI: https://doi.org/10.1007/BF01951733

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