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Malarial parasites complete sporogony in axenic mosquitoes

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Summary

To obtain sporogonic stages of malaria free from microbial contaminants for in vitro studies,Anopheles stephensi were reared under sterile conditions using a mosquito cell line as larval food. The adult females, kept in sterile humidified containers and allowed to engorge on parasitemic hamsters, supported the sporogonic development of the rodent malarial parasitePlasmodium berghei. In 10 experiments, the proportion of infected mosquitoes varied from 0 to 92%, and the geometric mean number of oocysts per female mosquito from 2.5 to 58,6, with a range of 1 to 548. The average number of salivary gland sporozoites per infected mosquito was determined by direct sporozoite counts in the pooled homogenate of the thoraces of all female mosquitoes. In five experiments, it varied from 2.7×103 to 9.0×103. The sterile sporozoites, harvested on day 19 or 20 after the infective blood meal, were as infective for rodents as nonsterile ones.

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References

  1. Boorman, J., Nature, Lond.213 (1967) 197; Rosales-Ronquillo, M. C., Simons, R. W., and Silverman, P. H., Ann. ent. Soc. Am.66 (1973) 949; Hamilton, D. R., and Bradley, R. E., Sr, J. Invert. Pathol.30 (1977) 318.

    Article  CAS  PubMed  Google Scholar 

  2. Sneller, V.-P., and Dadd, R. H., Exp. Parasit.51 (1981) 169.

    Article  CAS  PubMed  Google Scholar 

  3. Munderloh, U. G., and Kurtti, T. J. part of this work was presented as a poster at the 2nd International Conference on Malaria and Babesiosis, Annecy, France, September 1983.

  4. Vanderberg, J. P., Nussenzweig, R. S., and Most, H., J. Parasit.54 (1968) 1009.

    Article  CAS  PubMed  Google Scholar 

  5. Munderloh, U. G., Kurtti, T. J., and Maramorosch, K., J. Parasit.68 (1982) 1085.

    Article  CAS  PubMed  Google Scholar 

  6. Warhurst, D. C., and Folwell, R. O., Ann. trop. Med. Parasit.62 (1968) 349.

    Article  CAS  PubMed  Google Scholar 

  7. Schneider, I., and Vanderberg, J. P., in: Malaria, vol. 2, p. 235. Ed. J. P. Kreier, Academic press, New York 1980.

    Google Scholar 

  8. Weiss, M. M., and Vanderberg, J. P., J. Parasit.63 (1977) 932.

    Article  CAS  PubMed  Google Scholar 

  9. Clements, A. N., The Physiology of Mosquitoes, p. 154. Pergamon Press, New York 1963.

    Google Scholar 

  10. Gass, R. F., Acta trop.34 (1977) 127.

    CAS  PubMed  Google Scholar 

  11. Gass, R. F., Acta trop.36 (1979) 323.

    CAS  PubMed  Google Scholar 

  12. Gass, R. F., and Yeates, R. A., Acta trop.36 (1979) 243.

    CAS  PubMed  Google Scholar 

  13. Maier, W. A., Z. ParasitKde41 (1973) 11.

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Waksman Institute of Microbiology, Rutgers University, P.O. Box 759, 08854, Piscataway, New Jersey, USA

    U. G. Munderloh & T. J. Kurtti

Authors
  1. U. G. Munderloh
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  2. T. J. Kurtti
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Additional information

Supported in part by Public Health Service research grant AI 18345 from the National Institute of Allergy and Infectious Diseases, by a grant from the Agency of International Development DSPE-5542-G-SS-3042-00, and by a Charles and Johanna Busch award.

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Munderloh, U.G., Kurtti, T.J. Malarial parasites complete sporogony in axenic mosquitoes. Experientia 41, 1205–1207 (1985). https://doi.org/10.1007/BF01951731

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