Skip to main content
SpringerLink
Log in

Isolation and characterization of hemagglutinins from the sponge Dysidea herbacea

  • Short Communications
  • Published:
Experientia Aims and scope Submit manuscript

Summary

The spongeDysidea herbacea (Keller) was found to possess hemagglutinins. The major component, DHA-I, is a protein with a mol.wt of 26,000, which dissociates into subunits of equal size (14,000). It contains large amounts of glutamic acid and aspartic acid residues, but no half-cystine, methionine or histidine residues. DHA-I reacted with rabbit and human AB0 erythrocytes. D-galactose and lactose were effective inhibitors of DHA-I. The sponge also contained a minor component(s) which reacted preferentially with rabbit erythrocytes but not with human AB0 erythrocytes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Sharon, N., and Lis, H., Science177 (1972) 949.

    Article  CAS  PubMed  Google Scholar 

  2. Lis, H., and Sharon, N., A. Rev. Biochem.42 (1973) 541.

    Article  CAS  Google Scholar 

  3. Nicolson, G.L., Biochim. biophys. Acta458 (1976) 1.

    CAS  PubMed  Google Scholar 

  4. Gold, E. R., and Balding, Pl, Receptor-specific proteins, Plant and Animal Lectins, Excerpta Medica, Amsterdam 1975.

    Google Scholar 

  5. Dodd, R.Y., MacLennan, A.P., and Hawkins, D.C., Vox Sang.15 (1968) 386.

    Article  CAS  PubMed  Google Scholar 

  6. Bretting, H., and Kabat, E.A., Biochemistry15 (1976) 3228.

    Article  CAS  PubMed  Google Scholar 

  7. Bretting, H., Kabat, E.A., and Pereira, M.E.A., Biochemistry15 (1976) 5029.

    Article  CAS  PubMed  Google Scholar 

  8. Vaith, P., Uhlenbruck, G., Muller, W.E.G., and Holz, G., Devl comp. Immun.3 (1979) 399.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Fisheries Sciences, Kitasato University, Sanriku-cho, 022-01, Iwate, Japan

    H. Kamiya, K. Muramoto, T. Hoshino & U. Raj

  2. Mukaishima Marine Biological Station, Hiroshima University, 722, Mukaishima, Hiroshima, Japan

    H. Kamiya, K. Muramoto, T. Hoshino & U. Raj

  3. Institute of Marine Resources, The University of the South Pacific, Suva, Fiji

    H. Kamiya, K. Muramoto, T. Hoshino & U. Raj

Authors
  1. H. Kamiya
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Muramoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. Hoshino
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. U. Raj
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Acknowledgment. We thank Dr. M. Yamazaki, Faculty of Pharmaceutical Sciences, Teikyo University, for testing mitogenic activity ofDysidea agglutinins. This study was partly supported by a grant-in-aid for Overseas Scientific Survey from the Ministry of Education, Science and Culture, Japan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kamiya, H., Muramoto, K., Hoshino, T. et al. Isolation and characterization of hemagglutinins from the sponge Dysidea herbacea. Experientia 41, 1201–1202 (1985). https://doi.org/10.1007/BF01951729

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01951729

Key words

Search

Navigation