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Experientia

, Volume 41, Issue 9, pp 1177–1178 | Cite as

Role of calcium in the histamine release induced by D-galactosamine from rat mast cells

  • S. C. Sharma
  • O. P. Gulati
Short Communications

Summary

Rat peritoneal mast cells were isolated and purified by differential centrifugation in Ficoll. Cells pooled from three to four rats were suspended at approximately 106 cells/ml in a buffered salt solution and incubated for 1 h at 37°C in 300 μl volumes in the absence or presence (9×10−4 M) of calcium chloride. Addition of D-galactosamine hydrochloride (DGM; 2.8×10−4 M) caused (in addition to basal release) a mean ±SEM percent histamine release of 15.7±5.2 in the presence of Ca++ and 19±4.9 in the absence of Ca++ (p>0.05). It is suggested that D-galactosamine does not require extracellular Ca++ for the release of histamine from the rat mast cell.

Key words

Mast cells histamine D-galactosamine extracellular Ca++ 

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Copyright information

© Birkhäuser Verlag 1985

Authors and Affiliations

  • S. C. Sharma
    • 1
    • 2
  • O. P. Gulati
    • 1
    • 2
  1. 1.Department of PharmacologyTrinity College and Medical SchoolDublin-2(Ireland)
  2. 2.Unit of Pharmacology and BiochemistryZyma SANyon(Switzerland)

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