, Volume 41, Issue 9, pp 1177–1178 | Cite as

Role of calcium in the histamine release induced by D-galactosamine from rat mast cells

  • S. C. Sharma
  • O. P. Gulati
Short Communications


Rat peritoneal mast cells were isolated and purified by differential centrifugation in Ficoll. Cells pooled from three to four rats were suspended at approximately 106 cells/ml in a buffered salt solution and incubated for 1 h at 37°C in 300 μl volumes in the absence or presence (9×10−4 M) of calcium chloride. Addition of D-galactosamine hydrochloride (DGM; 2.8×10−4 M) caused (in addition to basal release) a mean ±SEM percent histamine release of 15.7±5.2 in the presence of Ca++ and 19±4.9 in the absence of Ca++ (p>0.05). It is suggested that D-galactosamine does not require extracellular Ca++ for the release of histamine from the rat mast cell.

Key words

Mast cells histamine D-galactosamine extracellular Ca++ 


  1. 3.
    Kazimierczak, W., and Diamant, B., Prog. Allergy24 (1978) 295.PubMedGoogle Scholar
  2. 4.
    Foreman, J. C., Hallet, M. B., and Mongar, J. C., J. Physiol.271 (1977) 193.PubMedGoogle Scholar
  3. 5.
    Douglas, W. W., and Ueda, Y., J. Physiol.234 (1973) 97P.Google Scholar
  4. 6.
    Atkinson, G., Ennis, M., and Pearce F. L., Br. J. Pharmac.65 (1979) 395.Google Scholar
  5. 7.
    Sharma, S. C., and Gulati, O. P., Blbl. Anat.20 (1981) 467.Google Scholar
  6. 8.
    Gulati, O. P., Sharma, S. C., and Hammersen, F., Archs int. Pharmacodyn. Ther.263 (1983) 272.Google Scholar
  7. 9.
    Cooper, P. H., and Stanworth, D. R., Meth. Cell Biol.14 (1976) 365.Google Scholar
  8. 10.
    Moodley, I., Monger, J. C., and Foreman, J. C., Eur. J. Pharmac.83 (1982) 69.CrossRefGoogle Scholar
  9. 11.
    Shore, P. A., Burkhalter, A., and Cohn, V. H., J. Pharmac. exp. Ther.127 (1959) 182.Google Scholar
  10. 12.
    Pearce, F. L., Ennis, M., Truneh, A., and White, J. R., Agents Actions11 (1981) 51.PubMedGoogle Scholar
  11. 13.
    Ennis, M., Pearce, F. L., and Weston, P. M., Br. J. Pharmac.70 (1980) 329.Google Scholar
  12. 14.
    Monger, J. L., and Schild, H. O., J. Physiol.140 (1958) 272.PubMedGoogle Scholar
  13. 15.
    Foster, A. B., Adv. Carbohydrates Chem. Biochem.12 (1957) 81.Google Scholar
  14. 16.
    Bloom, G. D., and Hagemark, O., Acta physiol. scand.71 (1967) 257.PubMedGoogle Scholar
  15. 17.
    Chakravarti, N., Nature194 (1962) 1182.PubMedGoogle Scholar
  16. 18.
    Moussatche, H., in: Handbk exp. Pharmac.18 (1966) 645.Google Scholar

Copyright information

© Birkhäuser Verlag 1985

Authors and Affiliations

  • S. C. Sharma
    • 1
    • 2
  • O. P. Gulati
    • 1
    • 2
  1. 1.Department of PharmacologyTrinity College and Medical SchoolDublin-2(Ireland)
  2. 2.Unit of Pharmacology and BiochemistryZyma SANyon(Switzerland)

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