Summary
Free amino acid composition of the intestinal contents, intestinal cells and hemolymph has been determined in larvae of the mothPhilosamia cynthia. From the hemolymph/lumen concentration ratio, an active transport could be inferred for neutral and basic amino acids. The values of cell/lumen and hemolymph/cell ratios suggested that the active step in the transport mechanism could be localized at the luminal pole of the enterocyte for neutral amino acids (except aromatic amino acids) and at the basolateral pole of the enterocyte for basic amino acids (except arginine).
References
Giordana, B., Sacchi, V.F., and Hanozet, G.M., Biochim. biophys. Acta692 (1982) 81.
Parenti, P., Giordana, B., Sacchi, V.F., Hanozet, G.M., and Guerritore, A., J. exp. Biol., in press (1985).
Jeuniaux, C., Duchateau-Bosson, G., and Florkin, M., Archs int. Physiol. Biochim.69 (1961) 617.
Florkin, M., and Jeuniaux, C., in: The Physiology of Insecta, vol. 5, p. 255. Ed. M. Rockstein, Academic Press, New York 1974.
Hanozet, G.M., Giordana, B., and Sacchi, V.F., Biochim. biophys. Acta596 (1980) 481.
Sacchi, V.F., Hanozet, G.M., and Giordana, B., J. exp. Biol.108 (1984) 327.
Hanozet, G.M., Giordana, B., Parenti, P., and Guerritore, A., J. Membrane Biol.81 (1984) 233.
Lund, P., in: Methods of Enzymatic Analysis, vol. 4, p. 1719. Ed. H.U. Bergmayer. Verlag Chemie/Academic Press, Weinheim 1974.
Giordana, B., and Sacchi, V.F., Comp. Biochem. Physiol.59A (1978) 17.
Wyatt, G.R., Loughheed, T.C., and Wyatt, S.S., J. gen. Physiol.39 (1956) 853.
Boctor, I.Z., and Salem S.I., Comp. Biochem. Physiol.45B (1973) 785.
Terra, W.R., Ferreira, C., and Santos, C.D., Comp. Biochem. Physiol.73A (1982) 373.
Ito, T., and Inokuchi, T., J. Insect Physiol.27 (1981) 447.
Nedergaard, S., in: Transport of Ions and Water in Animals, p. 239. Eds B.L. Gupta, R.B. Moreton, J.L. Oschman and B.J. Wall. Academic Press, London 1973.
Monticelli, G., and Giordana, B., Atti Acad. naz. Lincei73 (1982) 181.
Author information
Authors and Affiliations
Additional information
This work was supported by grants from Italian Consiglio Nazionale delle Ricerche and from Ministero della Pubblica Istruzione, Rome. The authors are indebted to Prof. V. Capraro for helpful discussion.
Rights and permissions
About this article
Cite this article
Parenti, P., Cidaria, D., Hanozet, G.M. et al. Free amino acid composition of the intestinal contents, intestinal cells and hemolymph ofPhilosamia cynthia larvae. Experientia 41, 1158–1160 (1985). https://doi.org/10.1007/BF01951706
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01951706