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Inhibition of sarcolemmal Na, K, Mg ATPase from the guinea pig heart is not compatible with a homogeneous population of non-interacting ouabain receptors

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Summary

The inhibition of sarcolemmal Na, K, Mg ATPase from the guinea pig heart by ouabain was evaluated with a coupled enzyme assay. Models of negative cooperativity and of two independent receptors fitted the inhibition data equally well. The analysis was not compatible with a homogeneous population of non-interacting ouabain receptors.

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References

  1. Taniguchi, K., and Iida, S., Biochim. biophys. Acta288 (1972) 98.

    Article  CAS  PubMed  Google Scholar 

  2. Erdmann, E., and Schoner, W., Biochim. biophys. Acta330 (1973) 302.

    Article  CAS  PubMed  Google Scholar 

  3. Hansen, O., Biochim. biophys. Acta433 (1976) 383.

    Article  CAS  Google Scholar 

  4. Onji, T., and Liu, M.-S., Archs Biochem. Biophys.207 (1981) 148.

    Article  CAS  Google Scholar 

  5. Werdan, K., Wagenknecht, B., Zwissler, B., Brown, L., Krawietz, W., and Erdmann, E., Biochem. Pharmac.33 (1984) 1873.

    Article  CAS  Google Scholar 

  6. Erdmann, E., Philipp, G., and Scholz, H., Biochem. Pharmac.29 (1980) 3219.

    Article  CAS  Google Scholar 

  7. Ebner, F., Naunyn-Schmiedebergs Arch. Pharmak.325 (1984) R49.

    Google Scholar 

  8. Jones, L. R., Besch, H. R., Fleming, J. W., McConnanphey, M. M., and Watanabe, A. M., J. biol. Chem.254 (1979) 530.

    Article  CAS  PubMed  Google Scholar 

  9. Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J., J. biol. Chem.193 (1951) 265.

    Article  CAS  PubMed  Google Scholar 

  10. Schwartz, A., Allen, J. C., and Harigaya, S., J. Pharmac. exp. Ther.158 (1969) 31.

    Google Scholar 

  11. Erdmann, E., Philipp, G., and Tanner, G., Biochim. biophys. Acta455 (1976) 287.

    Article  CAS  PubMed  Google Scholar 

  12. Segel, I. H., Enzyme Kinetics. John Wiley & Sons, New York, Chichester, Brisbane, Toronto 1975.

    Google Scholar 

  13. Fiske, C. H., Subbarow, Y., J. biol. Chem.66 (1925) 375.

    Article  CAS  Google Scholar 

  14. Noel, F., and Godfraind, T., Biochem. Pharmac.33 (1984) 47.

    Article  CAS  Google Scholar 

  15. Mansier, P., and Lelievre, L. G., Nature300 (1982) 535.

    Article  CAS  PubMed  Google Scholar 

  16. Sweadner, K. J., J. biol. Chem.254 (1979) 6060.

    Article  CAS  PubMed  Google Scholar 

Download references

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Authors and Affiliations

  1. Institut für Pharmakologie und Toxikologie der Technischen Universität München, Biedersteiner Strasse 29, D-8000, München 40, Federal Republic of Germany

    F. Ebner & G. Schönsteiner

Authors
  1. F. Ebner
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  2. G. Schönsteiner
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Acknowledgments. The technical assistance of P. Mayr and G. Ruhland is gratefully acknowledged.

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Ebner, F., Schönsteiner, G. Inhibition of sarcolemmal Na, K, Mg ATPase from the guinea pig heart is not compatible with a homogeneous population of non-interacting ouabain receptors. Experientia 41, 1147–1149 (1985). https://doi.org/10.1007/BF01951701

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