Testosterone secretion by Mongolian gerbil interstitial cells during short-term incubation depends on androgen precursors and serum proteins but not on gonadotrophins
Interstitial cells from the testes of the Mongolian gerbil have been used to investigate the effects of serum proteins on testosterone production stimulated by hCG and steroidal precursors. Short-term incubation of interstitial cells with progesterone or DHEA resulted in a rapid increase of testosterone secretion; this effect was even more pronounced in the presence of calf serum. On the other hand, addition of hCG (10 mIU) had no significant effect on testosterone release during the 30-min incubation. These results demonstrate that the magnitude of the steroidogenic response of short-term incubated interstitial cells is a complex function, mainly of precursor concentrations and binding capacities of serum proteins but not of gonadotrophins.
Key wordsInterstitial cells Mongolian gerbil in vitro secretion testosterone steroidal precursors serum proteins
Unable to display preview. Download preview PDF.
- 8.Lichtenberg, V., and Pahnke, V. G., Acta endocr., Suppl.202 (1976) 54.Google Scholar
- 12.Probst, B., and Fenske, M., Comp. Biochem. Physiol.85 A (1986a) 263–271.Google Scholar
- 18.Fenske, M., J. steroid Biochem., submitted.Google Scholar
- 19.Zeillinger, R., and Spona, J., Endocr. exp.18 (1984) 141.Google Scholar
- 20.Kime, D. E., Vinson, G. P., Major, P. W., and Kilpatrick, R., in: General, Comparative and Clinical Endocrinology of the Adrenal Cortex, p. 183. Eds I. Chester-Jones and I. W. Henderson. Academic Press. London 1980.Google Scholar