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Adenylate cyclase activation by trypsin in KB cell cultures

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Summary

Trypsin commonly used for cell dispersion increases adenylate cyclase activity of KB cells. It acts on catalytic receptors, since the apparent Km for ATP is lowered, and it alters the dependence of adenylate cyclase on Mg++ ions.

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References

  1. J. Otten, G. Johnson and I. Pastan Biochem. biophys. Res. Commun.44, 1192 (1971).

    Article  CAS  PubMed  Google Scholar 

  2. E. Rozengurt and A. Pardee, J. Cell Physiol.80, 273 (1972).

    Article  CAS  PubMed  Google Scholar 

  3. B. M. Bombik and M. M. Burger, Exp. Cell Res.80, 88 (1973).

    Article  CAS  PubMed  Google Scholar 

  4. A. Guiraud-Simplot and L. Colobert, C. r. Acad. Sci., Paris283, 93 (1976).

    CAS  Google Scholar 

  5. A. A. White and T. V. Zenzer, Analyt. Biochem.41, 372 (1971).

    Article  CAS  PubMed  Google Scholar 

  6. T. Russel and I. Pastan, J. biol. Chem.248, 5835 (1973).

    Article  Google Scholar 

  7. J. Otten, G. S. Johnson and I. Pastan, J. biol. Chem.247, 7082 (1972).

    Article  CAS  PubMed  Google Scholar 

  8. M. M. Burger, B. M. Bombik, B. M. Breckenridge and J. R. Sheppard, Nature New Biol.239, 161 (1972).

    Article  CAS  PubMed  Google Scholar 

  9. W. L. Ryan, N. A. Short and G. L. Curtis, Proc. Soc. exp. Biol. Med.150, 699 (1975).

    Article  CAS  PubMed  Google Scholar 

  10. H. J. Phillips and J. E. Terryberry, Exp. Cell Res.13, 341 (1957).

    Article  CAS  PubMed  Google Scholar 

  11. H. J. Phillips, Can. J. Biochem.45, 1495 (1967).

    Article  CAS  PubMed  Google Scholar 

  12. R. E. Scott, L. T. Furch and J. H. Kersey, Proc. nat. Acad. Sci. USA70, 3631 (1973).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. S. Venuta and H. Rubin, Proc. nat. Acad. Sci., USA70, 653 (1973).

    Article  CAS  PubMed  Google Scholar 

  14. W. B. Anderson, G. S. Johnson and I. Pastan, Proc. nat. Acad. Sci. USA70, 1055 (1973).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. W. B. Anderson, E. Lovelace and I. Pastan, Biochem. biophys. Res. Commun.52, 1293 (1973).

    Article  CAS  PubMed  Google Scholar 

  16. M. Yoshida, M. Owada and K. Toyoshima, Virology63, 68 (1975).

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Chimie Biologique et Médicale, Université Claude-Bernard, 8, avenue Rockefeller, F-69373, Lyon Cédex 2, France

    Annie Guiraud-Simplot & L. Colobert

Authors
  1. Annie Guiraud-Simplot
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  2. L. Colobert
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Guiraud-Simplot, A., Colobert, L. Adenylate cyclase activation by trypsin in KB cell cultures. Experientia 33, 899–901 (1977). https://doi.org/10.1007/BF01951270

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