Summary
Activity of the (Ca−Mg) ATPase of human red blood cell membranes is highly dependent on the specific buffer used in the ATPase assay. Activity is highest in histidine and/or imidazole buffers and is lowest in HEPES buffer.
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This research was supported by Public Health Service Grants AH-16436 and GM 00109-16. We wish to acknowledge the useful suggestions of Thomas R. Hinds and the excellent clerical assistance of Ms Laurie Asher.
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Farrance, M.L., Vincenzi, F.F. (Ca−Mg) ATPase activity of human erythrocyte membranes: Influence of incubation buffer. Experientia 33, 865–866 (1977). https://doi.org/10.1007/BF01951248
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DOI: https://doi.org/10.1007/BF01951248