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Experientia

, Volume 45, Issue 11–12, pp 1013–1018 | Cite as

Further evidence for the existence of a bottleneck in the metabolism ofSaccharomyces cerevisiae

  • L. C. M. Auberson
  • C. V. Ramseier
  • I. W. Marison
  • U. von Stockar
Multi-Author Review

Summary

The growth physiology ofSaccharomyces cerevisiae strains H1022 and Whi2+ has been studied in aerobic batch and continuous (chemostat) cultures. Results from the measurement of biomass and medium components (off-line) together with oxygen, carbon dioxide and heat measurements (on-line) have been used in an attempt to explore the existence of ‘overflow’ or ‘bottleneck’ metabolism as opposed to catabolite repression (Crabtree effect) in these strains. Chemostat experiments indicated that specific oxygen uptake rate (qo2) was linearly related to the dilution rate (D) at values below the critical dilution rate (D crit ), becoming constant aboveD crit , which is in agreement with the bottleneck theory. However, batch culture experiments indicated negligible oxygen consumption during the initial glucose growth phase, the culture exhibiting purely anaerobic metabolism. The bottleneck theory would propose thatq O 2, has a constant (maximum) value under these conditions. The results presented here suggest that while the bottleneck theory can be adequately used to describe chemostat growth ofS. cerevisiae, some other control mechanism must be operating under conditions of high glucose concentrations, such as those initially prevailing in the batch culture experiments.

Key words

Bottleneck theory calorimetry chemostat Crabtree effect glucose effect S. cerevisiae yeast metabolism 

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References

  1. 1.
    Barford, J. P., and Hall, R. J., A mathematical model for the aerobic growth ofSaccharomyces cerevisiae with a saturated respiratory capacity. Biotechnol. Bioeng.23 (1981) 1735–1762.Google Scholar
  2. 2.
    Käppeli, O., Petrik, M., and Fiechter, A., Transient responses ofSaccharomyces cerevisiae to a change of the growth-limiting nutrient in continuous culture. J. gen. Microbiol.131 (1985) 47–52.Google Scholar
  3. 3.
    Käppeli, O., Arreguin, M., and Rieger, M., The respirative breakdown of glucose bySaccharomyces cerevisiae: an assessment of a physiological state. J. gen. Microbiol.131 (1985) 1411–1416.Google Scholar
  4. 4.
    Käppeli, O., Regulation of carbon metabolism inSaccharomyces cerevisiae and related yeasts. Adv. Microbiol. Physiol.28 (1986) 181–209.Google Scholar
  5. 5.
    Marison, I. W., and von Stockar, U., A novel bench-scale calorimeter for biological process development work. Thermochim. Acta85 (1985) 493–496.Google Scholar
  6. 6.
    Marison, I. W., and von Stockar, U., The application of a novel heat flux calorimeter for studying growth ofEscherichia coli W in aerobic batch culture. Biotechnol. Bioeng.28 (1986) 1780–1793.Google Scholar
  7. 7.
    Petrik, M., Käppeli, O., and Fiechter, A., An expanded concept for the glucose effect in the yeastSaccharomyces uvarum: Involvement of short-and long-term regulation. J. gen. Microbiol.129 (1983) 43–49.Google Scholar
  8. 8.
    Polakis, E. S., Bartley, W., and Meek, G. A., Changes in the activities of respiratory enzymes during the aerobic growth of yeast on different carbon sources. Biochem. J.97 (1965) 298.Google Scholar
  9. 9.
    Rieger, M., Käppeli, O., and Fiechter, A., The respiratory capacity ofSaccharomyces cerevisiae, in: Current Development in Yeast Research. Eds G. G. Stewart and I. Russel. Pergamon Press, Toronto 1981.Google Scholar
  10. 10.
    Rieger, M., Käppeli, O., and Fiechter, A., The role of limited respiration in the incomplete oxidation of glucose bySaccharomyces cerevisiae. J. gen. Microbiol.129 (1983) 653–661.Google Scholar
  11. 11.
    Sonnleitner, B., and Käppeli, O., Growth ofSaccharomyces cerevisiae is controlled by its limited respiratory capacity: Formulation and verfication of a hypothesis. Biotechnol. Bioeng.28 (1986) 927–937.Google Scholar
  12. 12.
    von Stockar, U., and Birou, B., The heat generated by yeast cultures with a mixed metabolism in the transition between respiration and fermentation. Biotechnol. Bioeng.34 (1989) 86–101.Google Scholar

Copyright information

© Birkhäuser Verlag 1989

Authors and Affiliations

  • L. C. M. Auberson
    • 1
  • C. V. Ramseier
    • 1
  • I. W. Marison
    • 1
  • U. von Stockar
    • 1
  1. 1.Institute of Chemical EngineeringSwiss Federal Institute of TechnologyLausanneSwitzerland

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