, Volume 45, Issue 11–12, pp 1013–1018 | Cite as

Further evidence for the existence of a bottleneck in the metabolism ofSaccharomyces cerevisiae

  • L. C. M. Auberson
  • C. V. Ramseier
  • I. W. Marison
  • U. von Stockar
Multi-Author Review


The growth physiology ofSaccharomyces cerevisiae strains H1022 and Whi2+ has been studied in aerobic batch and continuous (chemostat) cultures. Results from the measurement of biomass and medium components (off-line) together with oxygen, carbon dioxide and heat measurements (on-line) have been used in an attempt to explore the existence of ‘overflow’ or ‘bottleneck’ metabolism as opposed to catabolite repression (Crabtree effect) in these strains. Chemostat experiments indicated that specific oxygen uptake rate (qo2) was linearly related to the dilution rate (D) at values below the critical dilution rate (D crit ), becoming constant aboveD crit , which is in agreement with the bottleneck theory. However, batch culture experiments indicated negligible oxygen consumption during the initial glucose growth phase, the culture exhibiting purely anaerobic metabolism. The bottleneck theory would propose thatq O 2, has a constant (maximum) value under these conditions. The results presented here suggest that while the bottleneck theory can be adequately used to describe chemostat growth ofS. cerevisiae, some other control mechanism must be operating under conditions of high glucose concentrations, such as those initially prevailing in the batch culture experiments.

Key words

Bottleneck theory calorimetry chemostat Crabtree effect glucose effect S. cerevisiae yeast metabolism 


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Copyright information

© Birkhäuser Verlag 1989

Authors and Affiliations

  • L. C. M. Auberson
    • 1
  • C. V. Ramseier
    • 1
  • I. W. Marison
    • 1
  • U. von Stockar
    • 1
  1. 1.Institute of Chemical EngineeringSwiss Federal Institute of TechnologyLausanneSwitzerland

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