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Comparison of chemiluminescence and absorptiometry in enzyme immunoassays for protein quantification

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Summary

The use of chemiluminescence in competitive binding assays for human serum albumin human alphafetoprotein and human immunoglobulin G and in double antibody sandwich enzyme immunoassays for cytomegalovirus and herpes simplex virus increased the sensitivity of the detection of antigen or antibody 16- to 95-fold above that obtained by conventional absorptiometric methods.

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Authors and Affiliations

  1. Department of Laboratory Medicine, Yale University School of Medicine, 06510, New Haven, Connecticut, USA

    A. D. Pronovost & A. Baumgarten

  2. Virology Laboratory, Veterans Administration Medical Center, 06516, West Haven, Connecticut, USA

    A. D. Pronovost & A. Baumgarten

Authors
  1. A. D. Pronovost
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  2. A. Baumgarten
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Additional information

Acknowledgments. This study was supported by Public Health Service research training grant T-32-AD 07018 from the Institute of Allergy and Infectious Diseases. To whom reprint requests should be addressed

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Pronovost, A.D., Baumgarten, A. Comparison of chemiluminescence and absorptiometry in enzyme immunoassays for protein quantification. Experientia 38, 304–306 (1982). https://doi.org/10.1007/BF01949356

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