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Experientia

, Volume 37, Issue 12, pp 1270–1271 | Cite as

Photodynamic studies on acid ribonuclease from pea cotyledons

  • P. S. Phillips
  • Carol A. Phillips
Specialia
  • 7 Downloads

Summary

In crude extracts, pea cotyledon acid ribonuclease is not inactivated by photodynamic treatment, but after 150-fold purification it is markedly inactivated when illuminated in the presence of rose bengal at pH 7.1. Data suggests that histidine photo-oxidation reduces catalytic activity.

Keywords

Purification Catalytic Activity Histidine Crude Extract Rose Bengal 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    D. Spikes and M. L. MacKnight, Ann. N. Y. Acad. Sci.171, 149 (1970).Google Scholar
  2. 2.
    J. S. Bellin and C. A. Yankus, Archs Biochem. Biophys.123, 18 (1968).CrossRefGoogle Scholar
  3. 3.
    J. A. Bryant, S. C. Greenway and G. A. West, Planta130, 141 (1976).CrossRefGoogle Scholar
  4. 4.
    B. I. S. Srivastava, Biochim. biophys. Acta169, 534 (1968).PubMedGoogle Scholar
  5. 5.
    G. Jori, G. Galiazzo, A. M. Tamburro and E. Scoffone, J. biol. Chem.245, 3375 (1970).PubMedGoogle Scholar
  6. 6.
    D. G. Smyth, W. H. Stein and S. Moore, J. biol. Chem.238, 227 (1963).Google Scholar
  7. 7.
    D. Findlay, D. Herries, A. P. Mathias, B. R. Rabin and C. A. Ross, Biochem. J.85, 152 (1962).Google Scholar
  8. 8.
    G. Jori, Int. Protein Res.1, 289 (1969).Google Scholar
  9. 9.
    A. M. Crestfield, W. H. Stein and S. Moore, J. biol. Chem.238, 2421 (1963).PubMedGoogle Scholar
  10. 10.
    F. M. Richards and H. W. Wyckoff, in: The Enzymes, vol. IV, p. 647. Ed. P. D. Boyer. Academic Press, New York 1971.Google Scholar

Copyright information

© Birkhäuser Verlag 1981

Authors and Affiliations

  • P. S. Phillips
    • 1
  • Carol A. Phillips
    • 1
  1. 1.Harestone ValleyCaterham CollegeCaterham(England)

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