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Improved sensitivity for detection and quantitation of glycoproteins on polyacrylamide gels

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Summary

A simple and sensitive technique for staining glycoproteins on polyacrylamide gels utilizing periodic acid-Schiff (PAS) reagent is described. The novelty of this technique is an additional incubation of the PAS stained gels in an ethanolic solution of metabisulfite. The treatment results in an about 20-fold increase in the intensitiy of bands and the color is stable for at least 1 week at room temperature. Furthermore, the integrated optical density of the bands is proportional to the amount of glycoproteins. Amounts as small as 0.2 μg of fetuin can be conveniently determined. As compared to other PAS staining methods, this procedure offers a remarkably improved sensitivity for detection and quantitation of glycoproteins following their electrophoretic separation on polyacrylamide gels.

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References

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Authors and Affiliations

  1. Institute of Medical Physiology C, and Institute of Neuropathology, University of Copenhagen, 3 Blegdamsvej, DK-2200, Copenhagen N., Denmark

    G. Konat, H. Offner & J. Mellah

Authors
  1. G. Konat
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  2. H. Offner
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  3. J. Mellah
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Additional information

Author for correspondence and reprint requests: G.K., Department of Neurobiology and Anatomy, The University of Texas Medical School, P.O. Box 20708, Houston, Texas 77025, USA.

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Konat, G., Offner, H. & Mellah, J. Improved sensitivity for detection and quantitation of glycoproteins on polyacrylamide gels. Experientia 40, 303–304 (1984). https://doi.org/10.1007/BF01947594

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  • DOI: https://doi.org/10.1007/BF01947594

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