Experientia

, Volume 40, Issue 3, pp 276–277 | Cite as

Rat testes interstitial cell nuclei exhibit three distinct receptors for retinoic acid

  • F. O. Cope
  • K. L. Knox
  • R. C. HallJr
Short Communications

Summary

Purified nuclei from rat testes interstitial cells were incubated with an equimolar complex of [3H]retinoic acid and purified cellular retinoic acid-binding protein (cRABP) and with ATP. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and radiofluorographic analysis of the nuclear fractions indicated the presence of 3 highly labeled receptors for retinoic acid which were distinct from cRABP. These data demonstrate that retinoic acid binds to 3 novel nuclear acceptors of which cRABP does not appear to be a part.

Keywords

Sodium Electrophoresis Dodecyl Sodium Dodecyl Retinoic Acid 

References

  1. 3.
    Lotan, R., Biochim. biophys. Acta605 (1980) 33.PubMedGoogle Scholar
  2. 4.
    De Luca, L., in: Vitamins and Hormones, vol. 35, p. 1. Academic Press, New York 1977.Google Scholar
  3. 5.
    Shidoji, Y., and De Luca, L. M., Biochem. J.206 (1981) 529.Google Scholar
  4. 6.
    Takase, S., Ong, D. E., and Chytil, F., Proc. natl Acad. Sci. USA76 (1979) 2204.PubMedGoogle Scholar
  5. 7.
    Liau, G., Ong, D. E., and Chytil, F., J. Cell Biol.91 (1981) 63.CrossRefPubMedGoogle Scholar
  6. 8.
    Mancino, V., Nasca, M., Bono, A., Leutskaya, Z. K., and Fais, D., IRCS Med. Sci.8 (1980) 115.Google Scholar
  7. 9.
    Steinberger, A., in: Methods in Enzymology, vol. 39. p. 283. Academic Press, New York 1975.Google Scholar
  8. 10.
    Salhanick, A. L., and Terner, C., Biol. Reprod.21 (1979) 293.PubMedGoogle Scholar
  9. 11.
    Fleisher, S., and Kervina, M., in: Methods in Enzymology, vol. 31, p. 6. Academic Press, New York 1974.Google Scholar
  10. 12.
    Bendetto, J. P., Martel, M. B., and Got, R., Biochim. biophys. Acta603 (1980) 245.PubMedGoogle Scholar
  11. 13.
    Berthillier, G., Bendetto, J. P., and Got, R., Biochim. biophys. Acta603 (1980) 245.PubMedGoogle Scholar
  12. 14.
    Dewald, B., Dulaney, J. T., and Touster, O., in: Methods in Enzymology, vol. 32, p. 82. Academic Press, New York 1974.Google Scholar

Copyright information

© Birkhäuser Verlag 1984

Authors and Affiliations

  • F. O. Cope
    • 1
  • K. L. Knox
    • 1
  • R. C. HallJr
    • 1
  1. 1.The Department of Nutritional SciencesThe University of ConnecticutStorrsUSA

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