Slice cultures of cerebellar, hippocampal and hypothalamic tissue
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Cerebellar, hippocampal and hypothalamic slices prepared from newborn and 7-day-old rats were cultured by means of the roller-tube technique. Identification of cells was made easier by the fact that at least part of the characteristic cytoarchitecture of the tissue was preserved in vitro. Cerebellar Purkinje cells and neurones of the deep cerebellar nuclei were recognized on the basis of their size, their location within the culture and their dendritic arborization. Pyramidal cells of all hippocampal subfields displayed their characteristic basal and apical dendritic trees with numerous spinous processes. Hippocampal granule cells gave rise to a monopolar dendritic arbor; their axons terminated in the dentate hilus and CA3 region. Golgi-like immuniperoxidase staining allowed localization of groups of neurophysin-positive neurones in slices prepared from the anterior hypothalamus. These neurones, bilaterally bordering the third ventricle, usually displayed a simple dendritic arborization and fine beaded axons.—Cultivation of brain slices prepared from young rats offers particular advantages in that the cultured cells are organized in an organotypic monolayer and individual living neurones may be directly visualized.
KeywordsPurkinje Cell Granule Cell Spinous Process Cerebellar Nucleus Dendritic Tree
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- 5.Angevine, J.B. Jr, Time of neuron origins in the hippocampal region. An autoradiographic study in the mouse. Exp. Neurol., suppl.2 (1965) 1–70.Google Scholar
- 9.Baertschi, A.J., Bény, J.L., and Gähwiler, B., The hypothalamo-hypophysial system in long-term tissue culture. in: Vasopressin, Corticoliberin and Opiomelancortins, pp. 259–271. Eds. A.J. Baertschi and J.J. Dreifuss. Plenum Press, London 1982.Google Scholar
- 13.Chan-Palay, V., Cerebellar Dentate Nucleus. Organization, Cytology and Transmitters, Springer-Verlag, Berlin 1977.Google Scholar
- 18.Gähwiler, B.H., Hypothalamic magnocellular neurones in culture, in: Vasopressin, Corticoliberin and Opiomelanocortins, pp. 129–135. Eds. A.J. Baertschi and J.J. Dreifuss. Academic Press, London 1982.Google Scholar
- 23.Hendelman, W.J., Marshall, K.C., Aggerwal, A.S., and Wojtovicz, J.M., Organization of pathways in cultures of mouse cerebellum, in: Cell, Tissue and Organ Cultures, pp. 539–555. Eds S. Fedoroff and L. Hertz. Academic Press, New York 1977.Google Scholar
- 30.Leontovich, T.A., The neurons of the magnocellular neurosecretory nuclei fo the dog's hypothalamus. J. Hirnforsch.11 (1969/70) 499–517.Google Scholar
- 36.Privat, A., Dendritic growth in vitro, in: Physiology and Pathology of Dendrites. Advances in Neurology, vol. 4, pp. 201–216. Ed. G.W. Kreutzberg. Raven Press, New York, 1975.Google Scholar
- 38.Sofroniew, M.V., Gähwiler, B.H., and Dreifuss, J.J., Cultured hypothalamic vasopressin (AVP), oxytocin (OT) and neurophysin (NPH) neurons examined by Golgi-like immunoperoxidase staining. Neuroscience7 (1982) S198-S199.Google Scholar
- 42.Zimmer, J., Development of the hippocampus and fascia dentata: morphological and histochemical aspects, in: Maturation of the Nervous System, pp. 171–189. Ed. M.A. Corner, Elsevier/North Holland Press, Amsterdam 1978.Google Scholar